二倍体马铃薯中SCF^(SLF)复合体的组分分析  

Component Analysis of SCF^(SLF) Complex in Diploid Potato

作　　者：高蒙 李富婷 魏湛林 张赛行 白如仟 尚轶 马玲 GAO Meng;LI Fu-ting;WEI Zhan-lin;ZHANG Sai-hang;BAI Ru-qian;SHANG Yi;MA Ling(College of Life Science,Yunnan Normal University,Kunming 650500;School of Energy and Environment Science,Yunnan Normal University,Kunming 650500)

机构地区：[1]云南师范大学生命科学学院,昆明650500 [2]云南师范大学能源与环境科学学院,昆明650500

出　　处：《生物技术通报》2022年第4期117-125,共9页Biotechnology Bulletin

基　　金：云南省教育厅科学研究基金项目(2020J0090);云南师范大学研究生科研创新基金(ysdyjs2020085)。

摘　　要：自交不亲和(self-incompatibility,SI)广泛存在于开花植物中,是防止近交促进杂交的一种途径,可有效维持物种的基因型多样性。车前科、茄科、蔷薇科等植物中的自交不亲和类型属于典型的配子体型自交不亲和(gametophytic selfincompatibility,GSI)。在GSI植物的杂交过程中,花粉管中的SCF^(SLF)复合体(Skp1/Cullin1/F-box)作为E3泛素连接酶,参与了花粉对异我型S-核糖核酸酶(S-RNase)的降解过程,是维持杂交亲和的关键因子。本研究通过整理GSI植物SCF^(SLF)复合体的研究进展,借助同源比对、进化树及蛋白序列分析等生物信息学分析手段,对二倍体马铃薯中SCF^(SLF)复合体的各个组分进行了初步分析,并对各组分基因的表达量和蛋白互作情况进行分析,最终确定了马铃薯花粉管中的SCF^(SLF)复合体可能是由Soltu.DM.06G001040,Soltu.DM.11G004330,Soltu.DM.06G022320以及相应的SLF蛋白组成的。Self-incompatibility(SI)exists widely in flowering plants,which is a way to prevent inbreeding and promote hybridization,and may effectively maintain the genotypic diversity of species.The type of SI in Plantaginaceae,Solanaceae,Rosaceae and other plants belongs to the typical gametophytic self-incompatibility(GSI).In the cross-pollination of GSI plants,the SCF^(SLF)complex(Skp1/Cullin1/F-box)in the pollen tube,as the E3 ubiquitin ligase,is involved in the degradation of S-RNase,which is the key factor to maintain the crossing compatibility.This study summarized the research progress of SCF^(SLF)complex in GSI plants,and preliminarily analyzed the components of SCF^(SLF)complex in diploid potato by homologous alignment,phylogenetic analysis and multiple protein sequence alignments.The gene expression and protein interaction of each component were analyzed,and it was preliminarily determined that the SCF^(SLF)complex in potato pollen tube may be composed of Soltu.DM.06G001040,Soltu.DM.11G004330,Soltu.DM.06G022320 and the corresponding SLF proteins.

关 键 词：SCF^(SLF)复合体 配子体型自交不亲和 马铃薯 

分 类 号：S532[农业科学—作物学]