血清miR-27b、miR-223水平与骨肉瘤患者化疗效果的相关性  

Correlation between serum miR-27b and miR-223 levels and the effect of chemotherapy in patients with osteosarcoma

作　　者：唐定波[1] 肖继伟[1] 张芹[1] 易颖 TANG Dingbo;XIAO Jiwei;ZHANG Qin;YI Ying(Department of Orthopedics,Sichuan Provincial Tumor Hospital,Sichuan Chengdu 610000,China)

机构地区：[1]四川省肿瘤医院骨科,四川成都610000

出　　处：《现代肿瘤医学》2022年第11期2029-2034,共6页Journal of Modern Oncology

基　　金：四川省卫生和计划生育科研课题资助项目(编号:17PJ212)。

摘　　要：目的:观察骨肉瘤患者血清微小RNA-27b、miR-223水平与化疗效果的相关性,指导未来临床对骨肉瘤患者实施合理的化疗。方法:选择我院2016年01月至2020年01月收治的241例骨肉瘤患者作为研究对象,均于化疗前检测血清miR-27b及miR-223水平,实施化疗治疗,分析血清miR-27b及miR-223水平与化疗效果的相关性。结果:241例骨肉瘤患者行化疗治疗后,总缓解率为59.75%;化疗后未缓解组肿瘤直径≥5 cm占比高于缓解组,化疗前血清miR-27b相对表达量高于缓解组,miR-223相对表达量低于缓解组,差异有统计学意义(P<0.05);两组其他基线资料比较,差异无统计学意义(P>0.05);经二元Logistic回归分析后建立多元回归模型,结果显示,骨肉瘤患者肿瘤直径、化疗前血清miR-27b及miR-223表达水平是化疗后未缓解的影响因素(OR>1,P<0.05);绘制受试者工作曲线(ROC)发现,化疗前血清miR-27b及miR-223水平预测化疗后未缓解的曲线下面积(area under curve,AUC)均>0.80,有一定预测性能。结论:骨肉瘤患者血清miR-27b、miR-223水平与化疗效果存在密切相关,化疗前血清miR-27b过表达及miR-223表达下调均可能提示化疗效果不佳,可将二者作为骨肉瘤患者化疗效果的有效预测因子,并根据评估结果合理调整治疗方案,可能对提高化疗整体获益意义重大。Objective:To observe the correlation between serum microRNA-27b,miR-223 levels and chemotherapy effects in patients with osteosarcoma,to guide the future clinical implementation of rational chemotherapy for patients with osteosarcoma.Methods:241 patients with osteosarcoma admitted to our hospital from January 2016 to January 2020 were selected as the research subjects.The serum miR-27b and miR-223 levels were detected before chemotherapy,and were all performed chemotherapy.To analyze the correlation between serum miR-27b and miR-223 levels and chemotherapy effects.Results:After 241 patients with osteosarcoma treated with chemotherapy,the total remission rate was 59.75%.After chemotherapy,the proportion of tumor diameter≥5 cm in the non-remission group was higher than that in the remission group.The relative expression of serum miR-27b before chemotherapy was higher than that of the remission group.The relative expression of miR-223 was lower than that of the remission group,and the difference was significant statistically(P<0.05).Compared other baseline data between the two groups,the difference was not significant statistically(P>0.05).After binary logistic regression analysis,a multiple regression model was established.The results showed that tumor diameter in patients with osteosarcoma and the expression levels of miR-27b and miR-223 in serum before chemotherapy were the influencing factors of non-remission after chemotherapy(OR>1,P<0.05).The receiver operating curve(ROC)was drawn and found that the serum miR-27b and miR-223 levels before chemotherapy predicted the area under the curve(AUC)after chemotherapy were both>0.80,which had certain predictive performance.Conclusion:The serum miR-27b and miR-223 levels in patients with osteosarcoma are closely related to the effect of chemotherapy,the overexpression of serum miR-27b and the down-regulation of miR-223 expression before chemotherapy may indicate poor chemotherapy effect.Both can be used as effective predictors of the chemotherapy effect of patients wit

关 键 词：骨肉瘤 化疗 微小RNA-27b 微小RNA-223 肿瘤直径 肿瘤位置 

分 类 号：R738.1[医药卫生—肿瘤]