The role of Smad6 in immunity of the pearl oyster Pinctada fucata martensii  

作　　者：Yu SHI Xiaolan PAN Meng XU Huiru LIU Hanzhi XU Maoxian HE 

机构地区：[1]CAS Key Laboratory of Tropical Marine Bio-resources and Ecology,Guangdong Provincial Key Laboratory of Applied Marine Biology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China [2]Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou),Guangzhou 511458,China

出　　处：《Journal of Oceanology and Limnology》2022年第3期1135-1147,共13页海洋湖沼学报（英文）

基　　金：Supported by the Natural Science Foundation of Guangdong Province,China(No.2019A1515011968);the Key Special Project for Introduced Talents Team of the Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)(No.GML2019ZD0401);the Earmarked Fund for the Modern Agro-industry Technology Research System(No.CARS-49);the Science and Technology Planning Project of Guangdong Province,China(No.2020B1212060058)。

摘　　要：Inhibitory Smads(I-Smads),which belong to the Smad family and inhibit bone morphogenic protein 2(BMP2)signaling by a variety of mechanisms,can suppress innate immunity responses in vertebrates.However,there are no reports for the role of Smad6 in immunity in mollusks.In this study,we showed that Smad6 of the pearl oyster Pinctada fucata martensii was located in the Smad6 cluster of the phylogenetic tree;mRNA expression of Smad6 and Smad3 was up-regulated after lipopolysaccharide and polyinosinic:polycytidylic challenge;and transcript levels of Smad6 and Smad3 showed opposite patterns during wound healing.Under salinity stress,water inflow and outflow in the gills appear to be regulated by BMP2-Smads signals,and BMP2-Smads signaling may be closely related to the immune response.Our results indicate that Smad6 is involved in immunity,that it plays a positive role in the response to immune challenge and an inhibitory role during wound healing,and that Smad6 and Smad3 may work against each other.

关 键 词：SMAD6 BMP2-Smads signal pathway expression IMMUNITY Pinctada fucata martensii 

分 类 号：S917.4[农业科学—水产科学]