Taxonomy and toxin profi le of harmful benthic Prorocentrum(Dinophyceae)species from the Xisha Islands,South China Sea  

作　　者：Jian ZOU Qun LI Hui LIU Ying LIU Lifen HUANG Haiyan WU Jiangbing QIU Hua ZHANG Songhui LÜ 

机构地区：[1]Research Center of Harmful Algae and Marine Biology,College of Life Science and Technology,Jinan University,Guangzhou 510362,China [2]State Environmental Protection Key Laboratory of Drinking Water Source Management and Technology,Shenzhen Academy of Environmental Science,Shenzhen 510008,China [3]Yellow Sea Fisheries Research Institute,Qingdao 266071,China [4]College of Environmental Science and Engineering,Ocean University of China,Qingdao 266100,China [5]Shenzhen Academy of Environmental Science,Shenzhen 510008,China [6]Southern Marine Science and Engineering Guangdong Laboratory,Zhuhai 519000,China

出　　处：《Journal of Oceanology and Limnology》2022年第3期1171-1190,共20页海洋湖沼学报（英文）

基　　金：Supported by the National Natural Science Foundation of China(Nos.41876173,41606176,41576162,41606175);the Special Foundation for National Science and Technology Basic Research Program of China(Nos.2018FY100200,2018FY100100)。

摘　　要：Some benthic Prorocentrum can produce okadaic acid(OA)and dinophysistoxins(DTXs)that cause diarrheic shellfish poisoning(DSP)in humans.The diversity and toxin profi les(OA and DTXs)of benthic Prorocentrum were investigated in the Xisha Islands,South China Sea.The benthic Prorocentrum was identified by both morphological features and molecular phylogenies.Morphologies were examined by light,fluorescence,and scanning electron microscopy,and phylogenetic analyses were based on partial large subunit(LSU)rDNA and ITS1-5.8S-ITS2(ITS)region.Seven Prorocentrum species including P.borbonicum,P.caipirignum,P.concavum,P.elegans,P.cf.emarginatum,P.lima complex,and P.rhathymum were identified in Xisha Islands.Among them,P.borbonicum and P.elegans were recorded in Chinese waters for the first time.OA and DTXs contents of seven benthic Prorocentrum species were evaluated based on liquid chromatography-tandem mass spectrometry(LC-MS/MS).All Xisha Islands strains of P.lima complex produced OA at contents ranging from 1663 to 3816 fg/cell.P.caipirignum also generated OA at 407 fg/cell,but other five species had no detectable toxins.Besides,interestingly,two strains of P.lima complex produced DTX-1 only(74 and 183 fg/cell)and another two strains generated an isomer of OA and DTX-2.Our findings provided insight into the biodiversity of benthic Prorocentrum in the Xisha Islands and pointed out the potential risk of DSP in this area.

关 键 词：PROROCENTRUM diversity dinofl agellate okadaic acid dinophysistoxins Xisha Islands 

分 类 号：X17[环境科学与工程—环境科学] Q949.2[生物学—植物学]