产纤维素酶芽孢杆菌的鉴定与酶活力比较  

Identification and Enzyme Activity Comparison of Cellulase-producing Bacillus

作　　者：江学斌[1] 成雪鸿 马苗鹏[2] 邓仲晴 李嘉慧 JIANG Xuebin;CHENG Xuehong;MA Miaopeng;DENG Zhongqing;LI Jiahui(School of Life Science,Guangzhou University,Guangzhou 510006,China;College of Life Sciences,South China Agricultural University/Guangdong Key Laboratory of Protein Function and Regulation in Agricultural Organisms,Guangzhou 510642,China)

机构地区：[1]广州大学生命科学学院,广东广州510006 [2]华南农业大学生命科学学院/广东省农业生物蛋白质功能与调控重点实验室,广东广州510642

出　　处：《广东农业科学》2022年第4期116-122,共7页Guangdong Agricultural Sciences

摘　　要：【目的】从14株细菌中筛选产纤维素酶菌株,为新型饲料添加剂的开发提供材料基础。【方法】采用刚果红染色法从14株细菌中初步筛选出产纤维素酶菌株;对产纤维素酶菌株进行菌落形态观察和16S rDNA基因序列同源性分析鉴定;使用刚果红染色法比较各菌株降解纤维素的能力,用DNS法检测各菌株的纤维素酶活力。【结果】初步筛选出7株产纤维素酶菌株,经16S rDNA鉴定,其中4株为地衣芽孢杆菌、3株为枯草芽孢杆菌。刚果红染色法初步判定7株芽孢杆菌纤维素降解能力大小表现为LW006>LW005>LW004>LW002>LW007>LW003>LW001,其中菌株LW006(枯草芽孢杆菌)降解纤维素能力最强,其透明圈直径达22.5 mm;经DNS法测定,7株芽孢杆菌的纤维素酶活力大小表现为LW006>LW005>LW004>LW007>LW003>LW002>LW001,其中菌株LW006纤维素酶活力最高,酶活力为1.22(±0.07)U/mL。【结论】菌株LW006是相对高产纤维素酶的菌株,有望为新型饲料添加剂提供新的菌种资源。【Objective】The research was carried out to screen out celllulase-producing strains from 14 strains of bacteria in order to provide a material basis for the development of new feed additives.【Method】The cellulase-producing strains were screened out from 14 strains by Congo red staining.Then the colony morphologies of them were observed and 16S r DNA gene sequence homology was analyzed and identified.After comparing the ability of each strain to degrade cellulose by Congo red staining,the cellulase activity of each strain was detected by DNS method.【Result】Seven cellulase-producing strains were screened,including 4 strains of Bacillus licheniformis and 3 strains of Bacillus subtilis.Based on Congo red staining,it was preliminarily identified that the cellulose degradation ability of 7 Bacillus strains was showed as:LW006>LW005>LW004>LW002>LW007>LW003>LW001.Among them,strain LW006(B.subtilis)had the strongest ability to degrade cellulose,producing a clear circle with a diameter of 22.5 mm.The cellulase activities of 7 strains of Bacillus were determined by DNS method as follows:LW006>LW005>LW004>LW007>LW003>LW002>LW001.Among them,strain LW006(B.subtilis)showed the highest cellulase activity with 1.22(±0.07)U/mL(P<0.01).【Conclusion】Strain LW006 is a relatively high cellulase-producing strain,which is expected to provide new strain resources for new feed additives.

关 键 词：纤维素酶 纤维素降解菌 筛选 鉴定 芽孢杆菌 

分 类 号：S816.7[农业科学—饲料科学]