小鼠10%皮肤软组织缺损创伤后应激障碍抑郁样行为模型的构建及其发生机制探讨  

Construction and Mechanism of Depression-like Behavior Model of Post-traumatic Stress Disorder in Mice with 10%Skin and Soft Tissue Defects

作　　者：刘立柱 李超艺 林诗炜 曲野 LIU Lizhu;LI Chaoyi;LIN Shiwei;QU Ye(Department of Traumatology,the Second Affi liated Hospital of Hainan Medical College,Haikou 570311,Hainan,China)

机构地区：[1]海南医学院第二附属医院创伤外科,海南海口570311

出　　处：《中国美容医学》2022年第5期98-102,共5页Chinese Journal of Aesthetic Medicine

基　　金：海南省医药卫生科研项目(编号:2001320274A2008)。

摘　　要：目的:探究10%皮肤软组织缺损构建小鼠创伤后应激障碍抑郁样行为模型的可行性,并探讨其发生机制。方法:32只C57BL/6小鼠,雌雄各半,随机分为对照组-雄性,对照组-雌性,创伤后应激障碍-雄性,创伤后应激障碍-雌性,共四组。取创伤后应激障碍组小鼠,背部剃毛,去除背部约10%面积的皮肤软组织,深度达深筋膜层。记录小鼠体重变化,7 d后,取小鼠脑组织用于HE染色,使用ELISA试剂盒检测脑组织中单胺类神经递质5-HT、NE、DA与炎性细胞因子TNF-α、IL-6水平,采用Western blot检测小鼠脑组织中MAOA表达水平。结果:10%皮肤软组织缺损造成小鼠体重短期内迅速下降,与对照组相比,无论雄性还是雌性小鼠,糖水偏好摄取率、穿越平台次数显著减少,悬尾不动时间与强迫游泳不动时间显著增加,差异有统计学意义(P<0.05)。10%皮肤软组织缺损导致小鼠脑组织海马区神经元损伤,炎性细胞因子TNF-α、IL-6分泌水平增加,单胺类神经递质5-HT、NE、DA浓度下降,差异有统计学意义(P<0.05)。与对照组相比,无论雄性还是雌性小鼠脑组织中MAOA表达水平显著增加,差异有统计学意义(P<0.05)。结论:采用10%皮肤软组织缺损构建小鼠创伤后应激障碍抑郁样行为模型是可行的,其机制可能是通过增加MAOA表达,降低单胺类神经递质5-HT、NE、DA表达水平。Objective To explore the feasibility of constructing a depression-like behavior model of post-traumatic stress disorder(PTSD)in mice with 10%skin and soft tissue defects,and to explore its mechanism.Methods Thirty-two C57BL/6 mice,male and female in half,were randomly divided into the control group-male,the control group-female,the PTSD-male,and the PTSD-female.The mice in the post-traumatic stress disorder group were shaved on the back,and about 10%of the skin and soft tissue on the back was removed to the depth of deep fascia.Recorded the weight change of the mice.After 7 days,the mice were sacrificed and brains were taken for HE staining,ELISA kits were used to detect secretion of monoamine neurotransmitters 5-HT,NE,DA and infl ammatory cytokines TNF-α,IL-6 in the brain.Western blot was used to detect the expression level of MAOA in mouse brain tissue.Results 10%skin and soft tissue defects caused a rapid decrease in the weight of mice quickly.Compared with the Control group,no matter male or female mice,the sucrose preference and the number of crossing platforms were significantly reduced,the time of tail suspension and forced swimming immobility signifi cantly increased,the diff erences were statistically signifi cant(P<0.05).In addition,10%skin and soft tissue defects caused neuronal damage in the hippocampus,increased secretion of infl ammatory cytokines TNF-αand IL-6(P<0.05),and monoamine neurotransmitters 5-HT,NE,DA concentration were decreased(P<0.05).Compared with the control group,the expression level of MAOA in the brain tissue of both male and female mice increased signifi cantly(P<0.05).Conclusion It is feasible to construct a depression-like behavior model of post-traumatic stress disorder in mice with 10%skin and soft tissue defects.The mechanism may be to increase the expression of MAOA and reduce the expression of monoamine neurotransmitters 5-HT,NE,DA.

关 键 词：皮肤软组织缺损 创伤后应激障碍 单胺氧化酶A 抑郁样行为 小鼠 动物模型 

分 类 号：R749.99[医药卫生—神经病学与精神病学]