miR-145通过靶向ABCE1发挥抗乳腺癌作用  

miR-145 exerts anti-breast cancer effects by targeting ABCE1

作　　者：陈双华 李荣[1,3] 周玉生[1,4] CHEN Shuang-hua;LI Rong;ZHOU Yu-sheng(Institute of Pharmacology,University of South China,Hengyang Hunan 421001;Hunan College of Traditional Chinese Medicine,Zhuzhou Hunan 412000;The Second Affiliated Hospital,University of South China,Hengyang Hunan 421001;Affiliated Nanhua Hospital,University of South China,Hengyang Hunan 421001)

机构地区：[1]南华大学药物药理研究所,湖南衡阳421001 [2]湖南中医药高等专科学校,湖南株洲412000 [3]南华大学附属第二医院,湖南衡阳421001 [4]南华大学附属南华医院,湖南衡阳421001

出　　处：《中南药学》2022年第5期1017-1022,共6页Central South Pharmacy

基　　金：湖南省卫生健康委员会重点指导项目(No.20201914)。

摘　　要：目的探讨miR-145靶向调节ABCE1的分子机制及其在抗乳腺癌细胞增殖中的作用。方法利用生物信息学方法筛选出靶向调节ABCE1的miR-145,取乳腺癌组织和癌旁组织检测ABCE1和miR-145的表达水平。通过在乳腺癌细胞MCF-7中转染miR-145 mimic和inhibitor,分析miR-145对ABCE1的蛋白表达及细胞凋亡的影响。采用荧光素酶报告基因实验验证miR-145对ABCE1 mRNA的靶向调节作用。结果在乳腺癌组织中ABCE1蛋白的表达明显高于癌旁组织,同时miR-145在癌组织中的含量显著降低。在MCF-7细胞中转染miR-145 mimic后能抑制ABCE1的表达,同时促进细胞凋亡。此外,在293T细胞中共转染miR-145 mimic和psiCHECK-ABCE1-3'UTR后,发现荧光素酶活性受到明显抑制。结论miR-145可通过靶向沉默ABCE1 mRNA来抑制乳腺癌细胞增殖,提示miR-145对ABCE1的调控可作为治疗乳腺癌的途径之一。Objective To determine the molecular mechanism of miR-145 targeting and regulating ABCE1 and its role in the anti-proliferation of breast cancer cells.Methods Bioinformatics methods were used to screen out miR-145 that targeted and regulated ABCE1.Breast cancer tissues and adjacent tissues were taken to detect the expression levels of ABCE1 and miR-145.By transfecting miR-145 mimic and inhibitor into breast cancer cell MCF-7,the effect of miR-145 on ABCE1 protein expression and cell apoptosis was analyzed.The luciferase reporter gene experiment was used to verify the targeted regulation of miR-145 on ABCE1 mRNA.Results The expression of ABCE1 protein in the breast cancer tissues was significantly higher than that in the adjacent tissues,and the content of miR-145 in the cancer tissues was reduced.Transfection of miR-145 mimic in MCF-7 cells inhibited the expression of ABCE1 and promoted the cell apoptosis.In addition,after co-transfection of miR-145 mimic and psiCHECK-ABCE1-3'UTR in 293T cells,luciferase activity was significantly inhibited.Conclusion MiR-145 can inhibit the proliferation of breast cancer cells by targeting ABCE1 mRNA silence,suggesting that the regulation of ABCE1 by miR-145 can be used as a treatment for breast cancer.

关 键 词：MIR-145 ABCE1 乳腺癌 乳腺癌细胞 

分 类 号：R737.9[医药卫生—肿瘤]