Essential oil from Saussurea costus inhibits proliferation and migration of Eca109 cells via mitochondrial apoptosis and STAT3 signaling  

作　　者：Xia-Fen Hu Wan-Xin Liu Ren Zhang Wei Zhang Chao Wang Meng Chen Rong Shu Xin-Zhou Yang Qiang Wang 

机构地区：[1]Institute of Infection,Immunology and Tumor Microenvironment,Hubei Province Key Laboratory of Occupational Hazard Identification and Control,Wuhan Asia General Hospital,Medical College,Wuhan University of Science and Technology,Wuhan,Hubei 430065,China [2]The Third People's Hospital of Hubei Province,Zhongshan Hospital of Hubei Province,Wuhan,Hubei 430030,China [3]School of Pharmaceutical Sciences,South-Central University for Nationalities,182 Minzu Road,Wuhan,Hubei 430074,China

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2022年第6期253-261,共9页亚太热带生物医学杂志（英文版）

基　　金：financially supported by the Scientific Research and Innovation Fund of Wuhan Asia General Hospital (Project No. WAGH-DSE-2022-005);2020 General Planning Fund Project for Humanities and Social Sciences of the Ministry of Education,China (Project No. 20YJA880053);Key Research Project of Philosophy and Social Sciences of Hubei Provincial Department of Education in 2020 (Project No. 20D026);WUST National Defence Pre-research Foundation,China (Project No. GF202003);Hubei Province Key Laboratory of Occupational Hazard Identification and Control,Wuhan University of Science and Technology (Project No. OHIC2020G05);National Undergraduate Innovation and Entrepreneurship Training Project (Project No. JCX201976)

摘　　要：Objective:To investigate the effect and its underlying molecular mechanisms of essential oil from Saussurea costus in esophageal cancer cell line Eca109.Methods:The chemical composition of essential oil from Saussurea costus was investigated by gas chromatography-mass spectrometry(GC-MS).The anti-proliferative,anti-migrative,and apoptotic effects of essential oil from Saussurea costus against Eca109 cells were analyzed.Moreover,the expression of proteins associated with cell cycle,metastasis,and apoptosis was determined.Results:GC-MS analysis showed that essential oil from Saussurea costus was predominantly comprised of sesquiterpenes.Saussurea costus essential oil inhibited the viability of Eca109 cells in a dose-and time-dependent manner with IC_(50) values of(24.29±1.49),(19.16±2.27)and(6.97±0.86)μg/mL at 12,24,and 48 h,respectively.The expression levels of target proteins in the cell cycle(phase G_(1)/S),including cyclin D1,p21,and p53,were affected by Saussurea costus essential oil.The essential oil also downregulated the expression of metastasis-related proteins MMP-9 and MMP-2.Moreover,it induced apoptosis of Eca109 cells through the mitochondrial pathway,as well as inhibition of STAT3 phosphorylation.Conclusions:The essential oil from Saussurea costus exhibited anti-proliferative,anti-migrative,and apoptotic effects on Eca109 cells,and could be further explored as a potential anti-esophageal cancer agent.

关 键 词：Saussurea costus Essential oil Gas chromatography-mass spectrometry Apoptosis Mitochondrial pathway STAT3 Esophageal cancer ANTI-PROLIFERATION Anti-migration ECA109 

分 类 号：R285[医药卫生—中药学]