血清hsa_circ_0000437对胃癌辅助诊断及预后判断的意义  

Significance of serum circRNA hsa_circ_0000437 in the auxiliary diagnosis and prognosis of gastric cancer

作　　者：申娴娟[1] 沈蕾[1] 马硕 戚菁[2] 鞠少卿[1] Shen Xianjuan;Shen Lei;Ma Shuo;Qi Jing;Ju Shaoqing(Laboratory Medicine Center,Nantong 226001,China;Research Center of Clinical Medicine,Affiliated Hospital of Nantong University,Nantong 226001,China)

机构地区：[1]南通大学附属医院医学检验中心,南通226001 [2]南通大学附属医院临床医学研究中心,南通226001

出　　处：《中华检验医学杂志》2022年第5期509-515,共7页Chinese Journal of Laboratory Medicine

基　　金：国家自然科学基金面上项目(81871720,82072363);南通市科技计划项目(MS12021002)。

摘　　要：目的探讨胃癌患者血清hsa_circ_0000437表达水平及其临床价值。方法选取2018年10月至2020年12月南通大学附属医院经病理确诊的80例胃癌患者(男57例,女23例);50例胃良性疾病患者(男28例,女22例)及80名健康对照者(男46例,女34例)。收集血清样本,同时收集上述胃癌患者中35例患者手术后血清样本。采用实时荧光定量PCR(RT-qPCR)方法检测血清hsa_circ_0000437表达水平。采用Mann-Whitney U检验比较不同组间、胃癌患者不同分期及手术前后血清hsa_circ_0000437水平的差异,采用χ²检验分析胃癌患者血清hsa_circ_0000437水平与其临床病理特征之间的相关性。化学发光法检测癌胚抗原(CEA)、糖类抗原199(CA199)、糖类抗原724(CA724)。运用受试者工作特征(ROC)曲线及其曲线下面积(AUC)评估hsa_circ_0000437、CEA、CA199以及CA724的诊断效能。Kaplan-Meier生存曲线分析hsa_circ_0000437表达水平与患者预后的关系。结果胃癌、胃良性疾病患者和健康对照者血清hsa_circ_0000437表达水平分别为2.252(1.235,4.765)、1.598(1.139,1.982)及1.000(0.818,1.385),胃癌患者高于胃良性疾病和健康对照者(P均<0.001),胃良性疾病患者高于健康对照者(P<0.001)。胃癌患者术后血清hsa_circ_0000437的表达水平低于手术前(P<0.001)。胃癌患者血清hsa_circ_0000437的表达水平在肿瘤T分期、N分期、肿瘤分化程度差异均有统计学意义(P=0.043、P=0.025和P=0.013)。胃癌患者区别于健康对照者,hsa_circ_0000437、CEA、CA199和CA724 AUC分别为0.863、0.619、657和0.608,4项指标联合检测AUC为0.892,敏感度高达97.5%(78/80)。Kaplan-Meier生存曲线显示血清hsa_circ_0000437高表达患者的总体生存率明显低于血清hsa_circ_0000437低表达患者(P=0.008)。结论hsa_circ_0000437可能是胃癌辅助诊断和预后判断的一个生物学指标。Objective To investigate the expression of hsa_circ_0000437 in the serum of patients with gastric cancer and its clinical value.Methods The serum samples from 80 patients(57 males and 23 females)with pathologically confirmed gastric cancer(GC),50 gastric benign disease(28 males and 22 females)and 80 healthy controls(46 males and 34 females)were collected from October 2018 to December 2020 in Affiliated Hospital of Nantong University.Serum samples from 35 of 80 gastric cancer patients after operation were collected.The expression of serum hsa_circ_0000437 was determined by real-time fluorescent quantitative PCR(RT-qPCR).Serum carcinoembryonic antigen(CEA),carbohydrate antigen 199(CA 199)and carbohydrate antigen 724(CA724)were determined by chemiluminescence method.Comparisons of serum hsa_circ_0000437 between groups were performed by Mann-Whitney U test.The correlation between serum expression of hsa_circ_0000437 in gastric cancer patients and its clinical pathological characteristics was performed byχ2 test.Receiver operating characteristic(ROC)curve and the area under the curve of ROC(AUC)were used to evaluate their diagnosis efficiency.Kaplan-Meier survival curve analysis was used to analyze the relationship between the expression level of serum hsa_circ_0000437 and the prognosis of patients.Results The relative expression of hsa_circ_0000437 in GC,gastric benign disease,healthy controls were 2.252(1.235,4.765),1.598(1.139,1.982)and 1.000(0.818,1.385)respectively.The relative expression of hsa_circ_0000437 in GC was significantly higher than that in gastric benign disease(P<0.001)and healthy controls(P<0.001).The difference between gastric benign disease and healthy controls was also statistically significant(P<0.001).The differences of serum hsa_circ_0000437 expression in GC patients between T stage,N stage,and tumor differentiation were statistically significant.The AUC of hsa_circ_0000437,CEA,CA199 and CA724 in GC patients were 0.863,0.619,657 and 0.608 respectively compared with healthy controls.The AUC of

关 键 词：胃肿瘤 环状RNA 实时荧光定量PCR 生物标志物 

分 类 号：R735.2[医药卫生—肿瘤]