不同浓度姜黄素对胃癌SGC-7901细胞增殖、自噬性凋亡和TGF-β/Smad信号通路的影响  

Effects of Different Concentrations of Curcumin on the Proliferation,Autophagic Apoptosis and TGF-β/Smad Signal Pathway of Gastric Cancer SGC-7901 Cells

作　　者：薛倩 刘鹏 李洪波 伍学强[1] 黄新春[1] XUE Qian;LIU Peng;LI Hong-bo;WUXue-qiang;HUANG Xin-chun(Department of Oncology,Beijing Aerospace General Hospital,Beijing,100076,China;Department of Oncology,People's Hospital Affiliated to Hebei Medical University/Shijiazhuang People's Hospital,Shijiazhuang,Hebei,050011,China)

机构地区：[1]北京航天总医院肿瘤科,北京100076 [2]河北医科大学附属人民医院/石家庄市人民医院肿瘤科,河北石家庄050011

出　　处：《现代生物医学进展》2022年第8期1424-1428,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81601184)。

摘　　要：目的:研究不同浓度姜黄素(Cur)体外对胃癌SGC-7901细胞增殖、自噬性凋亡和TGF-β/Smad信号通路的影响。方法:体外培养SGC-7901细胞,以不同浓度Cur作用于SGC-7901细胞。MTT法检测不同浓度的Cur对SGC-7901细胞增殖的影响。Hoechst 33258法观察不同浓度Cur对SGC-7901细胞凋亡影响,流式细胞仪检测细胞凋亡率。划痕实验检测不同浓度Cur对SGC-7901迁移能力的影响。免疫印迹法检测细胞凋亡相关蛋白NF-κB、自噬相关蛋白Beclin1、LC3Ⅱ及TGF-β/Smad信号通路蛋白TGF-β和p-smad2/3表达。结果:Cur能够抑制胃癌SGC-7901细胞的增殖和迁移,并且Cur对增殖和迁移的影响具有浓度依赖性。Cur能够促进胃癌SGC-7901细胞自噬性凋亡的发生,Cur浓度越高,SGC-7901细胞凋亡率越高(P<0.05)。Cur处理胃癌SGC-7901细胞后NF-κB、Beclin1、LC3Ⅱ表达明显升高,而TGF-β、p-smad2/3表达明显降低,且NF-κB、Beclin1、LC3Ⅱ、TGF-β和p-smad2/3的变化具有浓度依赖性。结论:Cur能够抑制胃癌SGC-7901细胞增殖和迁移并诱导自噬性凋亡的发生,其机制与促进NF-κB、Beclin1、LC3Ⅱ表达,抑制TGF-β/Smad信号通路激活有关。Objective:To study the effects of different concentrations of curcumin(Cur)on the proliferation,autophagic apoptosis and TGF-β/Smad signaling pathway of gastric cancer SGC-7901 cells in vitro.Methods:SGC-7901 cells were cultured in vitro,and SGC-7901 cells were treated with different concentrations of Cur.The MTT method was used to detect the effect of different concentrations of Cur on the proliferation of SGC-7901 cells.The Hoechst 33258 method was used to observe the effect of Cur on the apoptosis of SGC-7901 cells.Flow cytometry was used to detect the apoptosis rate.The effect of different concentration of Cur on the migration ability of SGC-7901 was detected by scratch test.The apoptosis-related protein NF-κB,autophagy-related protein Beclin1,LC3Ⅱand TGF-β/Smad signaling pathway proteins TGF-βand p-smad2/3 expression were detected by Western blotting.Results:Cur could inhibit the proliferation and migration of gastric cancer SGC-7901 cells,and the effect of Cur on the proliferation and migration was concentration-dependent.Cur could promote the occurrence of autophagic apoptosis of gastric cancer SGC-7901 cells,the higher the Cur concentration,the higher the apoptosis rate of SGC-7901 cells(P<0.05).After Cur treatment of SGC-7901 cells,the expression of NF-κB,Beclin1,LC3Ⅱwere significantly increased,while the expression of TGF-β,p-smad2/3 was significantly decreased,and the expression of NF-κB,Beclin1,LC3Ⅱ,TGF-βand p-smad2/3 changes were concentration-dependent.Conclusion:Cur can inhibit the proliferation and migration of gastric cancer SGC-7901 cells and induce autophagic apoptosis,the mechanism may be that Cur could promote the expression of NF-κB,Beclin1,LC3Ⅱand inhibit the activation of TGF-β/Smad signal pathway.

关 键 词：姜黄素 胃癌 SGC-7901细胞 TGF-Β/SMAD信号通路 增殖 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]