^(1)H-MR波谱检测腓肠肌细胞内脂质浓度用于早期诊断2型糖尿病模型大鼠周围神经病变  

Intramyocellular lipid concentration of gastrocnemius measured with ^(1)H-MR spectroscopy for early diagnosis of diabetic peripheral neuropathy in rat models of type 2 diabetes mellitus

作　　者：梁晓莹 肖叶玉 廖中曦 陈杰 刘燕飞 LIANG Xiaoying;XIAO Yeyu;LIAO Zhongxi;CHEN Jie;LIU Yanfei(Department of Imaging, the Second Affiliated Hospital of Shantou University Medical College, Shantou 515041, China;Department of Imaging, Guangzhou Hospital of Integrated Traditional and West Medicine, Guangzhou 510800, China;Department of Imaging, Yiyang Central Hospital, Yiyang 413000, China)

机构地区：[1]汕头大学医学院第二附属医院影像科,广东汕头515041 [2]广州市中西医结合医院影像科,广东广州510800 [3]益阳市中心医院影像科,湖南益阳413000

出　　处：《中国介入影像与治疗学》2022年第6期365-369,共5页Chinese Journal of Interventional Imaging and Therapy

基　　金：广东省科技创新战略专项(2018A0303070002);广东省中医药局中医药科研项目(20202141);广州市科技计划项目(202102080665)。

摘　　要：目的观察^(1)H-MR波谱(MRS)检测腓肠肌肌细胞内脂质(IMCL)浓度对早期诊断2型糖尿病(T2DM)模型大鼠周围神经病变(DPN)的价值。方法将30只雄性SD大鼠随机分为糖尿病(DM)组和正常组,每组15只。对DM组以高糖高脂饲养4周联合单次腹腔注射1%链脲佐菌素(STZ)溶液45 mg/kg体质量建立T2DM模型,正常组则以普通饲料饲养4周联合注射1%STZ柠檬酸-柠檬酸钠缓冲液作为对照。于建模成功后第4、8周采集2组大鼠右后肢腓肠肌^(1)H-MRS,检测其IMCL浓度;于建模成功后第8周处死动物,检测2组右坐骨神经运动神经传导速度(MNCV)和感觉神经传导速度(SNCV),之后行病理学检查,观察坐骨神经组织变化,判断是否发生DPN;评估腓肠肌IMCL浓度对早期诊断DPN的价值。结果DM组15只均成功建立T2DM大鼠模型,建模成功后每周DM组大鼠空腹血糖及体质量均高于正常组(P均<0.05)。建模成功后第4、8周,DM组大鼠右后肢腓肠肌IMCL浓度均显著高于正常组(P均<0.01),且建模成功后第8周DM组及正常组大鼠IMCL浓度均明显高于第4周(P均<0.01)。建模成功后第8周,DM组大鼠均发生DPN,其右坐骨神经MNCV及SNCV均明显低于正常组(P均<0.001);病理结果示神经纤维异常变化。结论^(1)H-MRS检测腓肠肌IMCL对早期诊断T2DM大鼠DPN具有一定价值。Objective To observe the value of intramyocellular lipid(IMCL)concentration of gastrocnemius measured with ^(1)H-MR spectroscopy(^(1)H-MRS)for early diagnosis of diabetic peripheral neuropathy(DPN)in rat models of type 2 diabetes mellitus(T2DM).Methods Thirty male SD rats were enrolled and randomly divided into diabetes mellitus(DM)group or normal group(each n=15).Rats in DM group were fed with high sugar and high fat for 4 weeks,combined with single intraperitoneal injection of 1%streptozotocin(STZ)solution of 45 mg/kg to establish T2DM models,while rats in normal group were fed with ordinary diet for 4 weeks,combined with injection of 1%STZ citric acid-sodium citrate buffer solution solution in same as controls.^(1)H-MRS of gastrocnemius muscle of the rats'right hind leg were collected at the 4th and the 8th week after success of modeling,and IMCL concentrations were detected.At the 8th week after success of modeling,the rats were sacrificed,then motor nerve conduction velocity(MNCV)and sensory nerve conduction velocity(SNCV)of the right sciatic nerves were measured.HE staining was performed,and the changes of sciatic nerve were observed to evaluate DPN or not.The value of gastrocnemius IMCL concentration for early diagnosis of DPN was analyzed.Results T2DM rat models were successfully established in DM group.After modeling,the weekly fasting blood glucose and body mass of rats in DM group were higher than those in normal group(both P<0.05).At the 4th and the 8th week after success of modeling,gastrocnemius IMCL concentration of rats in DM group were higher than those in normal group(both P<0.01),and gastrocnemius IMCL concentration at the 8th week after success of modeling in DM and normal groups were both higher than those at the 4th week(both P<0.01).At the 8th week after success of modeling,DPN occurred in all rats in DM group,MNCV and SNCV of right sciatic nerve were significantly lower than those in normal group(both P<0.001),and pathological results showed abnormal changes of nerve fibers.Conclusion IMCL

关 键 词：糖尿病 2型 肌 骨骼 脂肪组织 糖尿病神经病变 磁共振波谱 大鼠 

分 类 号：R-332[医药卫生] R445.2