N-糖基化修饰对异源蛋白在毕赤酵母中表达的作用研究进展  

Research Progress on the Effect of N-glycosylation on Production of Heterologous Proteins in Pichia pastoris

作　　者：韩铭海 王未鲜 于志海 HAN Ming-hai;WANG Wei-xian;YU Zhi-hai(Guizhou Institute of Technology,Guiyang,Guizhou 550000)

机构地区：[1]贵州理工学院,贵州贵阳550000

出　　处：《安徽农业科学》2022年第11期14-17,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(31760022);贵州省科学技术基金资助项目(黔科合基础〔2019〕1146号)。

摘　　要：毕赤酵母是目前工业上生产重组蛋白最常用的宿主之一,而蛋白高水平表达技术一直是该领域的研究热点。目前,成熟的分子生物学技术手段使改造N-糖基化修饰位点成为一种操作性较强的提高目标蛋白表达水平的策略。综述了毕赤酵母中蛋白N-糖链的结构、其介导的CNX循环和对蛋白折叠的作用以及通过N-糖基化修饰位点改造促进蛋白表达的应用研究。Pichia pastoris is one of the most popular eukaryotic hosts for producing heterologous proteins.However,increased secretion of certain proteins valued for their applications is essential in this field.Due to promoted techniques in molecule biology,engineering N-glycosylation sites of target proteins was easily manipulated and an effective way to increase their productions.This paper reviewed the structure of N-glycan of glycoproteins in Pichia pastoris,the effect of its participation in CNX cycle on protein folding,and engineering N-glycosylation sites of the target proteins for stimulating their productions.

关 键 词：毕赤酵母 N-糖基化修饰 异源蛋白 蛋白折叠 

分 类 号：Q78[生物学—分子生物学]