PRLR 基因InDel检测及其与陕北白绒山羊生长性状的关联分析  

InDel Detection of PRLR Gene and Its Association Analysis with Growth Traits of Shanbei White Cashmere Goats

作　　者：王聪亮 张政轩 付琪 魏宇新 白晶晶 宋晓越 李陇平 屈雷[1] 朱海鲸[1,2,3] WANG Cong-liang;ZHANG Zheng-xuan;FU Qi(Shaanxi Province Engineering&Technology Research Center of Cashmere Goats,Yulin University,Yulin,Shaanxi 719000)

机构地区：[1]榆林学院生命科学研究中心,陕西榆林719000 [2]陕西省“四主体一联合”肉羊工程技术校企联合研究中心,陕西神木719318 [3]陕西浩丽绒山羊科技发展有限公司,陕西子洲718499

出　　处：《安徽农业科学》2022年第11期85-88,93,共5页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目:(31702115);榆林学院高层次人才科研启动基金项目:(16GK06);陕西省农业科技创新与攻关项目项目(2016NY-106);国家现代农业产业园建设绒山羊产业重点项目(2020HX02)。

摘　　要：[目的]探究催乳素受体(prolactin receptor,PRLR)基因InDel突变对绒山羊种群生长特性的影响。[方法]以陕北白绒山羊为研究对象,采用PCR扩增和测序技术法检测分析陕北白绒山羊PRLR基因的多态性,利用独立样本t检验和单因素方差法分析PRLR基因不同基因型与陕北白绒山羊生长性状之间的相关性。[结果]在所检测的1176只陕北白绒山羊群体中,PRLR基因第2内含子存在1个16 bp InDel突变,形成了纯合插入型(II)、杂合型(ID)与纯合缺失型(DD)3种基因型;在育成羊群体中,该InDel突变与生长性状无显著关联(P>0.05),在成年羊群体中,InDel突变与管围性状显著相关(P<0.05);在育成羊和成年羊全部群体中,InDel突变仍与管围性状显著相关(P<0.05)。[结论]PRLR基因16 bp InDel突变可能会对陕北白绒山羊生长发育产生显著影响,该位点可作为陕北白绒山羊辅助育种的候选分子标记。[Objective]In order to investigate the effect of prolactin receptor(PRLR)gene InDel mutation on the growth characteristics of cashmere goat population.[Method]The Shanbei white cashmere goats were used as the research object,and used PCR amplification and direct sequencing techniques to detect the polymorphism of PRLR gene in Shanbei white cashmere goats,and analyzed the correlation between different genotypes of PRLR gene and growth traits of Shanbei white cashmere goats using independent sample t-test and one-way ANOVA.[Result]The results showed that there was one 16 bp InDel mutation in intron 2 of the PRLR gene in the 1176 Shanbei white cashmere goats population,and there were three genotypes:homozygous insertion type(II),heterozygous type(ID)and homozygous deletion type(DD),respectively.The InDel mutation was not significantly correlated with growth traits in the yearling goats population(P>0.05),and was significantly correlated with the cannon circumference trait in the adult goats population(P<0.05);In all of the goat population this InDel mutation was still significantly correlated with the cannon circumference trait(P<0.05).[Conclusion]The 16 bp InDel mutation in PRLR gene may impose significant effect on the growth and development of Shanbei white cashmere goats,and this locus can be used as a candidate molecular marker for the auxiliary breeding of high-yielding Shanbei white cashmere goats.

关 键 词：绒山羊 PRLR基因 INDEL 生长性状 关联分析 

分 类 号：S827[农业科学—畜牧学]