纳米磁酶水酶法在磁流化床中提取大豆油脂的数值模拟及应用  

Numerical simulation and application of nano-magnetic enzyme hydroenzymatic method for soybean oil and grease extraction in a magneto-fluidized bed

作　　者：吴非[1] 吴泽楠 孙秋月 王若桦 钟文雅 胡晓欢 杜晶[2] 于殿宇[1] Wu Fei;Wu Zenan;Sun Qiuyue;Wang Ruohua;Zhong Wenya;Hu Xiaohuan;Du Jing;Yu Dianyu(College of Food,Northeast Agricultural University,Harbin 150030,China;College of Arts and Sciences,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学食品学院,哈尔滨150030 [2]东北农业大学文理学院,哈尔滨150030

出　　处：《农业工程学报》2022年第6期302-311,共10页Transactions of the Chinese Society of Agricultural Engineering

基　　金：黑龙江省“百千万”工程科技重大专项“大豆生物基油料及蛋白制品关键技术集成与示范”项目(2020ZX08B01)。

摘　　要：为提高大豆油的提取率以及油的品质,该研究将游离纤维素酶固定在磁性高分子载体Fe_(3)O_(4)/SiOx-g-P(GMA)上,利用数值模拟确定磁流化床中水酶法提油的最佳参数,并将最佳参数应用在磁流化床中,通过单因素试验探究磁性固定化纤维素酶在磁流化床中水酶法提油的最佳工艺条件。结果表明:扫描电镜、粒径分析和红外光谱显示磁性固定化纤维素酶已固定在磁性高分子载体Fe_(3)O_(4)/SiOx-g-P(GMA)上,且具有较好的磁响应能力,与游离酶相比,磁性固定化纤维素酶提高了酶的耐热性和耐酸碱性。数值模拟得出磁场强度为0.034 T,流速为0.0041 m/s时,磁酶在磁流化床中可与大豆料液充分接触,有利于提高大豆油提取率。磁流化床中水酶法提油较优工艺为:酶添加量为1.2 mg/g,pH值为5,温度为55℃,反应时间120 min,此时大豆油提取率较优为90.3%。磁性固定化纤维素酶在磁流化床中可以连续使用12 h。该研究提高了大豆油提取率,与间歇反应相比,磁流化床大豆油提取率增加了6.1%。研究结果为后续磁流化床水酶法提油提供了理论依据。Soybean oil has been one of the most consumed cooking oil extracted from the seeds of the soybean.Aqueous and enzymatic processes can be widely expected for edible oil extraction in recent years.In this study,a series of numerical simulations were carried out to determine the optimum parameters for the aqueous and enzymatic oil extraction from a magnetic fluidized bed under the OpenFOAM software,in order to improve the oil quality,yield,and extraction rate of soybean.The free cellulase was also immobilized on a magnetic polymer carrier Fe_(3)O_(4)/SiOx-g-P(GMA)using the glutaraldehyde cross-linking protocols.The carrier was prepared using ethyl orthosilicate and 3-aminopropyl triethoxy,while,the Fe_(3)O_(4) nanoparticles were modified by silane.The magnetically immobilized cellulase with the better magnetic response was obtained for the subsequent flow of magnetizing application in the fluidized bed and the separation after the reaction.Scanning electron microscopy(SEM)images showed that the rough and uneven surface of the carriers significantly increased the surface area of the magnetically immobilized cellulase for the better adsorption of the enzyme.The particle size analysis and SEM images revealed that the particle size of magnetically immobilized cellulase was outstandingly enlarged with a more uniform morphology,compared with the carrier.Correspondingly,Fourier infrared spectroscopy(FIS)verified that the magnetically immobilized cellulase was immobilized on the polymer carrier Fe_(3)O_(4)/SiOx-g-P(GMA).The enzymatic properties of the immobilized cellulase and the free enzyme were evaluated to determine the optimum pH(5)and temperature(60℃)of the immobilized cellulase,indicating that the higher heat,acid,and alkali resistance of the cellulose than before immobilization.The discrete element method(DEM)was used to construct a two-dimensional numerical model of nano-magnetase particles.The OpenFOAM software was selected to obtain the best parameters of hydroenzymatic oil extraction in a magnetic fluidized be

关 键 词：油脂 提取 固定化纤维素酶 水酶法 磁流化床 数值模拟 

分 类 号：TS224.2[轻工技术与工程—粮食、油脂及植物蛋白工程]