Exogenous artificial DNA forms chromatin structure with active transcription in yeast  

作　　者：Jianting Zhou Chao Zhang Ran Wei Mingzhe Han Songduo Wang Kaiguang Yang Lihua Zhang Weigang Chen Mingzhang Wen Cheng Li Wei Tao Ying-Jin Yuan 

机构地区：[1]Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education),Tianjin University,Tianjin 300072,China [2]SynBio Research Platform,Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China [3]The MOE Key Laboratory of Cell Proliferation and Differentiation,School of Life Sciences,Peking University,Beijing 100871,China [4]CAS Key Laboratory of Separation Science for Analytical Chemistry,National Chromatographic R.&A.Center,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China [5]University of Chinese Academy of Sciences,Beijing 100049,China [6]School of Microelectronics,Tianjin University,Tianjin 300072,China

出　　处：《Science China(Life Sciences)》2022年第5期851-860,共10页中国科学（生命科学英文版）

基　　金：supported by the National Key Research and Development Program of China (2121YFA0909300);the National Natural Science Foundation of China (31861143017,21621004;31901019);the China Postdoctoral Science Foundation(2021M692389)。

摘　　要：Yeast artificial chromosomes(YACs) are important tools for sequencing,gene cloning,and transferring large quantities of genetic information.However,the structure and activity of YAC chromatin,as well as the unintended impacts of introducing foreign DNA sequences on DNA-associated biochemical events,have not been widely explored.Here,we showed that abundant genetic elements like TATA box and transcription factor-binding motifs occurred unintentionally in a previously reported datacarrying chromosome(d Chr).In addition,we used state-of-the-art sequencing technologies to comprehensively profile the genetic,epigenetic,transcriptional,and proteomic characteristics of the exogenous d Chr.We found that the data-carrying DNA formed active chromatin with high chromatin accessibility and H3K4 tri-methylation levels.The d Chr also displayed highly pervasive transcriptional ability and transcribed hundreds of noncoding RNAs.The results demonstrated that exogenous artificial chromosomes formed chromatin structures and did not remain as naked or loose plasmids.A better understanding of the YAC chromatin nature will improve our ability to design better data-storage chromosomes.

关 键 词：yeast artificial chromosome DNA storage EPIGENETICS chromatin accessibility histone H3K4 tri-methylation 

分 类 号：Q343.2[生物学—遗传学]