Surrogate production of genome-edited sperm from a different subfamily by spermatogonial stem cell transplantation  

作　　者：Fenghua Zhang Yongkang Hao Xianmei Li Yi Li Ding Ye Ru Zhang Xiaosi Wang Mudan He Houpeng Wang Zuoyan Zhu Yonghua Sun 

机构地区：[1]State Key Laboratory of Freshwater Ecology and Biotechnology,Hongshan Laboratory,Institute of Hydrobiology,Innovation Academy for Seed Design,Chinese Academy of Sciences,Wuhan 430072,China [2]College of Advanced Agricultural Sciences,University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Science China(Life Sciences)》2022年第5期969-987,共19页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China(32025037 and 31721005);the National Key R&D Project of China(2018YFA0801000 and 2018YFD0901205);Chinese Academy of Sciences(XDA24010108);State Key Laboratory of Freshwater Ecology and Biotechnology(2019FBZ05)。

摘　　要：The surrogate reproduction technique,such as inter-specific spermatogonial stem cells(SSCs)transplantation(SSCT),provides a powerful tool for production of gametes derived from endangered species or those with desirable traits.However,generation of genome-edited gametes from a different species or production of gametes from a phylogenetically distant species such as from a different subfamily,by SSCT,has not succeeded.Here,using two small cyprinid fishes from different subfamilies,Chinese rare minnow(gobiocypris rarus,for brief:Gr)and zebrafish(danio rerio),we successfully obtained Gr-derived genome-edited sperm in zebrafish by an optimized SSCT procedure.The transplanted Gr SSCs supported the host gonadal development and underwent normal spermatogenesis,resulting in a reconstructed fertile testis containing Gr spermatids and zebrafish testicular somatic cells.Interestingly,the surrogate spermatozoa resembled those of host zebrafish but not donor Gr in morphology and swimming behavior.When pou5f3 and chd knockout Gr SSCs were transplanted,Gr-derived genome-edited sperm was successfully produced in zebrafish.This is the first report demonstrating surrogate production of gametes from a different subfamily by SSCT,and surrogate production of genome-edited gametes from another species as well.This method is feasible to be applied to future breeding of commercial fish and livestock.

关 键 词：surrogate reproduction genome editing spermatogonial stem cell transplantation ZEBRAFISH Gobiocypris rarus 

分 类 号：S917.4[农业科学—水产科学]