PbPH5,an H^(+)P-ATPase on the tonoplast,is related to malic acid accumulation in pear fruit  

作　　者：SONG Jun-xing CHEN Ying-can LU Zhao-hui ZHAO Guang-ping WANG Xiao-li ZHAI Rui WANG Zhi-gang YANG Cheng-quan XU Ling-fei 

机构地区：[1]College of Horticulture,Northwest A&F University,Yangling 712100,P.R.China

出　　处：《Journal of Integrative Agriculture》2022年第6期1645-1657,共13页农业科学学报（英文版）

基　　金：funded by the National Key Research and Development Program of China (2019YFD1001400);the National Natural Science Foundation of China (31601715)

摘　　要：Organic acid content is one of the most important factors influencing fruit flavors.The predominant organic acid in most pear cultivars is malic acid,but the mechanism controlling its accumulation remains unclear.In this study,by comparing gene expression levels and organic acid contents,we found that the expression of PbPH5,which encodes a P_(3A)-ATPase,is highly correlated with malic acid accumulation in four different pear species,with correlation coefficients of 0.932**,0.656*,0.900**,and 0.518*(*,P<0.05;**,P<0.01)for Pyrus bretschneideri Rehd.,P.communis Linn.,P.pyrifolia Nakai.,and P.ussuriensis Maxim.,respectively.Moreover,the overexpression of PbPH5 in pear significantly increased the malic acid content.In contrast,silencing PbPH5 via RNA interference significantly decreased both its transcript level and the pear fruit malic acid content.A subcellular localization analysis indicated that PbPH5 is located in the tonoplast.Additionally,a phylogenetic analysis indicated that PbPH5 is a PH5 homolog gene that is clustered with the Petunia hybrida,Malus domestica,and Citrus reticulata genes.Considered together,these findings suggest that PbPH5 is a functionally conserved gene.Furthermore,the accumulation of malic acid in pear fruit is at least partly related to changes in the PbPH5 transcription levels.

关 键 词：PEAR P3A-ATPase PH5 homolog malic acid accumulation proton pump 

分 类 号：S661.2[农业科学—果树学]