不同GI与CBI组合饲粮对牦牛瘤胃和直肠细菌区系的影响  

Effects of Different Roughage Grading Index and Carbohydrate Balance Index Combinations Diets on Bacterial Flora in the Rumen and Rectum of Yaks

作　　者：郝文君 夏洪泽 焦洋 崔占鸿[1,2,3] 刘书杰 周磊[4] 周玉青 HAO Wenjun;XIA Hongze;JIAO Yang;CUI Zhanhong;LIU Shujie;ZHOU Lei;ZHOU Yuqing(Academy of Animal Husbandry and Veterinary Sciences,Qinghai University,Xining810016,China;Yak Engineering Technology Research Center of Qinghai Province,Xining 810016,China;Key Laboratory of Plateau Grazing Animal Nutrition and Feed Science of Qinghai Province,Xining 810016,China;Haibei Zangzu Autonomous Prefecture Plateau Ecological Animal Husbandry Science and Technology Demonstration Garden Administrative Committee,Haibei 812200,China)

机构地区：[1]青海大学畜牧兽医科学院,西宁810016 [2]青海省牦牛工程技术研究中心,西宁810016 [3]青海省高原放牧家畜动物营养与饲料科学重点实验室,西宁810016 [4]海北藏族自治州高原生态畜牧业科技示范园管委会,海北812200

出　　处：《中国畜牧兽医》2022年第5期1715-1726,共12页China Animal Husbandry & Veterinary Medicine

基　　金：青海省科技计划科技援青合作专项(2020-QY-220)。

摘　　要：【目的】本研究通过探讨不同粗饲料分级指数(GI)与碳水化合物平衡指数(CBI)组合饲粮对牦牛瘤胃和直肠细菌区系的影响,旨在为舍饲牦牛营养物质消化吸收的调控以及饲粮合理配制提供参考。【方法】选取3.5岁健康、体重相近的公牦牛12头分为3组,即玉米青贮-苜蓿干草-精料组(CAC组)、玉米青贮-小麦秸秆-精料组(CWC组)、玉米青贮-燕麦干草-精料组(COC组)。3组试验牛均采用全混合饲粮方式进行饲喂,试验期为28 d。试验结束时采集瘤胃液和直肠粪样,应用16S rRNA测序技术分析瘤胃和直肠细菌区系变化情况。【结果】对于瘤胃,①3组牦牛瘤胃细菌区系的Chao1指数、Ace指数、Simpson指数和Shannon指数差异均不显著(P>0.05);②PCoA分析结果显示,CAC与COC组的瘤胃细菌区系有明显的PC1差异(P<0.05);③在门水平上,CWC组牦牛瘤胃放线菌门与CAC、COC组存在显著差异(P<0.05);在属水平上,3组牦牛瘤胃细菌区系差异不显著(P>0.05);④瘤胃细菌基因功能预测结果显示,复制与修复、原核细胞群体、细胞信号传递、环境适应等KEGG二级代谢通路在CAC与COC组存在显著差异(P<0.05);复制与修复、转录、核苷酸代谢、脂质代谢、原核细胞群体和老化等KEGG二级代谢通路在CAC与CWC组存在显著差异(P<0.05);脂质代谢、其他氨基酸代谢和老化等KEGG二级代谢通路在CWC组和COC组存在显著差异(P<0.05)。对于直肠,①3组牦牛直肠细菌区系的Chao1指数、Ace指数、Simpson指数和Shannon指数差异均不显著(P>0.05);②PCoA分析结果显示,3组牦牛直肠细菌区系差异不显著(P>0.05);③在门水平上和属水平上3组牦牛直肠细菌区系差异均不显著(P>0.05);④直肠细菌基因功能预测结果显示,与能量代谢有关的KEGG二级代谢通路在CAC与COC组存在显著差异(P<0.05);膜转运、能量代谢、聚糖生物合成和代谢、辅助因子和维生素的新陈代谢、细胞运动、�【Objective】The aim of this study was to investigate the effects of different forage grade index(GI)and carbohydrate balance index(CBI)combined diets on rumen and rectal bacterial flora of yaks,and to provide theoretical reference value for the regulation of nutrient digestion and absorption and rational dietary allocation of yaks.【Method】Twelve healthy 3.5-year-old male yaks with similar weight were divided into three groups equally,which were corn silage-alfalfa hay-concentrate group(CAC),corn silage-wheat straw-concentrate group(CWC),and corn silage-oat hay-concentrate group(COC).All three groups of experimental yaks were fed with total mixed diet for 28 days.At the end of the experiment,rumen fluid and rectal stool samples were collected,and 16S rRNA sequencing technology was used to analyze the changes of rumen and rectum bacterial flora.【Result】For rumen,①The Chao1 index,Ace index,Simpson index and Shannon index of the rumen bacterial flora in the three groups were not significantly different(P>0.05).②PCoA analysis showed that there was significant PC1 difference in rumen flora between CAC and COC groups(P<0.05).③At the phylum level,the Actinobacteria in rumen of CWC group were significantly different from those in CAC and COC groups(P<0.05).At the genus level,there was no significant difference in rumen bacterial flora among the three groups(P>0.05).④Rumen bacterial gene function prediction showed that:The secondary differential KEGG metabolic pathways including replication and repair,cellular community-prokaryotes,cellular processes and signaling and environmental adaptation were significantly different in the rumen bacterial flora between CAC and COC groups(P<0.05),replication and repair,transcription,nucleotide metabolism,lipid metabolism,cellular community-prokaryotes and aging were significantly different between CAC and CWC groups(P<0.05),and lipid metabolism,metabolism of other amino acid and aging were significantly different between CWC and COC groups(P<0.05).For rectum,①The C

关 键 词：粗饲料分级指数(GI) 碳水化合物平衡指数(CBI) 牦牛 细菌区系 

分 类 号：S816.31[农业科学—饲料科学]