Lnc RNA CUDR敲低对肝癌侵袭、增殖和凋亡的影响  

Effects of Lnc RNA CUDR Knock-down on Invasion,Proliferation and Apoptosis of Hepatocellular Carcinoma

作　　者：王波[1] 金雯[1] 周健坤 陈良[2] 曹立宇[3] WANG Bo;JIN Wen;ZHOU Jian-kun;CHEN Liang;CAO Li-yu(Department of Madicaland Nursing,Tongling Polytechnic College,Tongling,Anhui,244061,China;Department of Hyperbaricoxygen,Thesecondpeople's Hospital of Hefei,Hefei,Anhui,230011,China;Department of Pathology,The First Affiliated Hospital of Anhui Medical University,Hefei,Anhui,230022,China)

机构地区：[1]铜陵职业技术学院医学护理系,安徽铜陵244061 [2]合肥市第二人民医院高压氧科 [3]安徽医科大学第一附属医院病理科

出　　处：《承德医学院学报》2022年第3期196-200,共5页Journal of Chengde Medical University

基　　金：安徽高校自然科学研究重点项目(KJ2020A0974);安徽高校优秀青年人才支持计划重点项目(gxydZD2021155);安徽省高等学校省级质量工程项目(2019cxtd043)。

摘　　要：目的探讨LncRNA CUDR敲低对肝癌细胞侵袭、增殖和凋亡的影响。方法采用CUDR shRNA慢病毒质粒转染SMMC-7721肝癌细胞系,分别设CUDR敲低组、对照组和空质粒组,采用RT-PCR方法检测CUDR shRNA转染效率;Transwell检测3组细胞的侵袭能力;瘤球成形实验检测细胞聚集、增殖能力;流式技术检测肝癌细胞凋亡率。结果RT-PCR检测结果显示,CUDR shRNA转染SMMC-7721肝癌细胞系后,与对照组、空质粒组相比,敲低组CUDR mRNA表达量显著下降(P<0.001);Transwell、瘤球成形实验和细胞凋亡实验结果显示,与对照组和空质粒组相比,CUDR敲低组肝癌细胞的侵袭与聚集能力显著下降且凋亡率提高(P<0.001)。结论敲低LncRNA CUDR表达可抑制HCC的侵袭和增殖,并促进肝癌细胞的凋亡,LncRNA CUDR具有成为治疗HCC潜在靶点的价值。Objective To investigate the effect of LncRNA CUDR knock-down on invasion,proliferation and apoptosis of hepatocellular carcinoma cells.Methods CUDR shRNA lentivirus plasmid was used to transfect SMMC-7721 hepatoma cell line.The CUDR knockdown group,control group and empty plasmid group were set up respectively.The transfection efficiency of CUDR shRNA was detected by RT-PCR method,the invasive ability of the three groups was detected by Transwell,the ability of cell aggregation and proliferation was detected by tumor ball formation test,and the apoptosis rate of hepatocellular carcinoma cells was detected by flow cytometry.Results The results of RT-PCR detection showed that the expression of CUDR mRNA in the knockdown group was significantly lower than that in the control group and empty plasmid group after CUDR shRNA transfection(P<0.001).The results of Transwell,tumor ball formation test and apoptosis assay showed that the invasion and aggregation abilities of hepatoma cells in the CUDR knockdown group were significantly lower than those in the control group and the empty plasmid group,and the apoptosis rate was increased in CUDR knockdown group(P<0.001).Conclusion Knocking down the expression of LncRNA CUDR can inhibit the invasion and proliferation of HCC and promote the apoptosis of hepatocellular carcinoma cells,LncRNA CUDR may be a potential therapeutic target for HCC.

关 键 词：肝细胞癌 长链非编码RNA CUDR 侵袭 增殖 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]