侵染凤仙花的菜豆金色花叶病毒的鉴定及基因组结构分析  

Molecular Identification and Sequence Analysis of Begomoviruses Infecting Impatiens balsamina

作　　者：钟静[1] 赵丽玲[1] 李婷婷[1] 张水英 陈越[1] 丁铭[1] ZHONG Jing;ZHAO Liling;LI Tingting;ZHANG Shuiying;CHEN Yue;DING Ming(Key Laboratory of Agricultural Biotechnology of Yunnan Province,Key Laboratory of the Southwestern Crop Gene Resources and Germplasm Innovation,Ministry of Agriculture and Rural Affairs,Institute of Biotechnology and Germplasm Resources,Yunnan Academy of Agricultural Sciences,Kunming 650223,China)

机构地区：[1]云南省农业科学院生物技术与种质资源研究所,云南省农业生物技术重点实验室,农业农村部西南农业基因资源与种质创制重点实验室,昆明650223

出　　处：《园艺学报》2022年第5期1136-1144,共9页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31960529);云南省农业基础研究联合专项(2018FG001-009);云南省基础研究计划项目(201801CC00115)。

摘　　要：在对云南省西双版纳州进行病毒病害调查时发现1株疑似菜豆金色花叶病毒属病毒侵染的凤仙花植株,其症状表现为叶片皱缩和花叶。利用菜豆金色花叶病毒属病毒简并引物进行PCR扩增、克隆和分析,获得YN6198-28和YN6198-31两条菜豆金色花叶病毒属病毒的DNA-A全长序列,长度均为2 763 bp,编码6个开放阅读框,其中YN6198-28核苷酸序列与越南丁香黄脉病毒(Ludwigia yellow vein Vietnam virus,LuYVVNV)相似性为94.90%,表明该分离物是LuYVVNV1个分离物。重组分析发现,YN6198-28是种间重组病毒,其主要亲本为丁香黄脉病毒(Ludwigia yellow vein virus,LuYVV),次要亲本为中国胜红蓟黄脉病毒(Ageratum yellow vein China virus,AYVCNV)。An investigation about diseases in Xishuangbanna Autonomous Prefecture,Yunnan Province identified an Impatiens balsamina plant with leaf shrinking and mosaic symptoms typical of begomovirus infections. To identify the possible virus associated with the disease,the sample was collected and detected by PCR with begomovirus degenerate primers. The amplicons were cloned and sequenced,and two sequences(YN6198-28 and YN6198-31)of 2 763 bp corresponding to begomovirus DNA-A genome with six open reading frames were identified from this sample. The YN6198-28 DNA-A sequence shares the highest identity(94.90%)with Ludwigia yellow vein Vietnam virus(LuYVVNV),which confirmed that the virus was an isolate of LuYVVNV. Recombination analysis revealed that the YN6198-28 isolate is a recombinant begomovirus derived from Ludwigia yellow vein virus and Ageratum yellow vein China virus.

关 键 词：凤仙花 菜豆金色花叶病毒属 重组病毒 

分 类 号：S681.1[农业科学—观赏园艺]