不同渗透压氯化钠溶液对不同种属动物红细胞的影响  

Effect of Different Osmotic Pressure Sodium Chloride Solutions on Erythrocytes of Different Species of Animals

作　　者：喻雨 任萌 孙双勇 李韶勇 董卫莉[1] Yu Yu;Ren Meng;Sun Shuangyong;Li Shaoyong;Dong Weili(School of Pharmacy Tianjin Medical University,Tianjin 300203;Pharmacology Department Tianjin Pharmaceuticals,Tianjin 300050,China)

机构地区：[1]天津医科大学药学院,天津300203 [2]天津医药集团有限公司,天津300050

出　　处：《广东化工》2022年第9期5-7,共3页Guangdong Chemical Industry

基　　金：天津市自然科学基金面上项目(NO:18JCYBJC89800)。

摘　　要：目的:探究四种不同种属来源的实验动物红细胞在不同浓度氯化钠溶液中的溶血情况。方法:分别使用日本大耳白兔,SD大鼠,豚鼠,绵羊的2%红细胞悬液在不同浓度的氯化钠溶液中孵育,再通过目视观察和全波长酶标仪测定吸光度判定实验结果。结果:在较低浓度氯化钠溶液(0.1%~1%)中,豚鼠红细胞发生溶血的氯化钠浓度范围最小,SD大鼠、日本大耳白兔次之,绵羊红细胞发生溶血的氯化钠浓度范围最大。在较高浓度氯化钠溶液(2%~20%)中,日本大耳白兔红细胞发生溶血的氯化钠浓度范围最小,大鼠次之,豚鼠和绵羊相似,红细胞发生溶血的氯化钠浓度范围较大。结论:在渗透压较低的溶液中,豚鼠红细胞对渗透压变化耐受性强;在渗透压较高的溶液中,日本大耳白兔红细胞对渗透压变化耐受性强;绵羊红细胞在高渗和低渗环境中对渗透压变化的耐受性都较差。Objective:Investigation of haemolysis of erythrocytes from four different species of experimental animals in different concentrations of sodium chloride solution.Methods:The results were determined by visual observation and absorbance measurement by full wavelength enzyme standardisation using 2%erythrocyte suspensions from rabbits,rats,guinea pigs and sheep incubated with different concentrations of NaCl.Results:Guinea pig erythrocytes are more tolerant to changes in osmolality in lower osmolality solutions;rabbit erythrocytes are more tolerant to changes in osmolality in lower osmolality solutions;sheep erythrocytes are less tolerant to changes in osmolality in both hypertonic and hypotonic environments.

关 键 词：体外溶血 氯化钠溶液 动物红细胞 渗透压 吸光度测定法 目视观察法 

分 类 号：TQ[化学工程]