载锌纳米线修饰钛表面的血管内皮细胞生物学行为研究  

The in vitro study on biological behaviors of endothelial cells on the titanium surface modified with zinc-containing nanowires

作　　者：苏珊 朱文卿 邱憬[1] SU Shan;ZHU Wenqing;QIU Jing(Department of Oral Implantology,Afiliated Stomatological Hospital,Jiangsu Prorince Key Laboratory of Oral Diseases,Jiangsu Prorince Engineering Research Center of Stomatological Translational Medicine,Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]南京医科大学附属口腔医院种植科,江苏省口腔疾病研究重点实验室,江苏省口腔转化医学工程研究中心,江苏南京210029

出　　处：《口腔医学》2022年第5期404-410,共7页Stomatology

基　　金：国家自然科学基金面上项目(81870799);江苏省重点研发计划(社会发展)(BE2019728);江苏高校“青蓝工程”中青年学术带头人项目。

摘　　要：目的探究载锌纳米线修饰钛表面对血管内皮细胞生物学行为的影响。方法纯钛试件抛光清洗后,通过酸蚀和碱热处理,在其表面制备纳米线(NW-Ti)和载锌纳米线(Zn-NW-Ti)结构。以光滑钛表面(cp-Ti)为对照组,纳米线、载锌纳米线修饰钛表面为实验组。通过扫描电镜、X射线光电子能谱(XPS)、接触角测量仪分析各组钛表面的微形貌、元素组成和亲水性;采用锌离子检测试剂盒检测载锌纳米线修饰钛表面释放的锌离子浓度;将人脐静脉内皮细胞系细胞(HUVECs)接种于各组试件表面,通过细胞粘附、增殖、迁移及实时荧光定量PCR实验,研究不同钛表面对人脐静脉内皮细胞行为的影响。结果载锌纳米线修饰钛表面为形貌均一的纳米线状拓扑结构,并含有微量锌元素,其释放的锌离子浓度约为1 mg/L。与其余两组相比,载锌纳米线修饰钛表面能促进人脐静脉内皮细胞的粘附、增殖和迁移,并上调血管生成相关因子HIF-1α、VEGF-A的表达水平。结论载锌纳米线修饰钛表面能促进血管内皮细胞粘附、增殖、迁移及相关功能基因的表达。Objective To investigate the effects of titanium surface modified with zinc-containing nanowire on the behavior of vascular endothelial cells. Methods After polishing up, acid-etching and alkali-heat treatment, the titanium surface modified with nanowires(NW-Ti) and zinc-containing nanowires(Zn-NW-Ti) were prepared on the commercially pure titanium(cp-Ti) surfaces. The cp-Ti were set as the control group, and two modified titanium surfaces were set as the experimental groups. Scanning electron microscopy(SEM), X-ray photoelectron spectroscopy(XPS) and contact angle meter were used to analyze the surface morphology, elemental compositions and hydrophi licity of three specimens. The concentration of zinc ions released from Zn-NW-Ti was tested by zinc ion releasing kit. Human umbilical vein endothelial cells(HUVECs) were seeded on different titanium surfaces to study the effects on angiogenesis properties by cell adhesion, proliferation, migration and real-time quantitative PCR assays. Results Trace zinc element was detected on Zn-NW-Ti, which presented a uniform topological structure of nanowires. The concentration of zinc ions released from Zn-NW-Ti was approximately 1 mg/L. Compared with the other two groups, Zn-NW-Ti could significantly promote the adhesion, proliferation, migration of HUVECs, and up-regulate mRNA levels of angiogenic factors: HIF-1α and VEGF-A. Conclusion The titanium surface modified with zinc-containing nanowires can promote the adhesion, proliferation, migration and angiogenic gene expression of vascular endothelial cells.

关 键 词：锌 纳米线 钛 内皮细胞 

分 类 号：R783.1[医药卫生—口腔医学]