Antibiofilm activity of 3,3'-diindolylmethane on Staphylococcus aureus and its disinfection on common food-contact surfaces  

作　　者：Hui Zhang Xiaomei Guo Lei Tian Na Wang Yuqing Li Ariel Kushmaro Robert Marks Qun Sun 

机构地区：[1]College of Biomass Science and Engineering,Sichuan University,Chengdu 610064,China [2]Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education,College of Life Sciences,Sichuan University,Chengdu 610064,China [3]Key Laboratory of Green Chemistry and Technology,Ministry of Education,College of Chemistry,Sichuan University,Chengdu 610064,China [4]State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Department of Cariology and Endodontics,West China Hospital of Stomatology,Sichuan University,Chengdu 610065,China [5]Avram and Stella Goldstein-Goren Department of Biotechnology Engineering,Ben-Gurion University of the Negev,Beer-Sheva 84105,Israel [6]The Ilse Katz Center for Meso and Nanoscale Science and Technology,Ben-Gurion University of the Negev,Beer-Sheva 84105,Israel

出　　处：《Food Science and Human Wellness》2022年第5期1222-1232,共11页食品科学与人类健康（英文）

基　　金：supported by the National Key Research and Development Projects(2019YFE0103800);Sichuan Science and Technology Program(2019YFH0113,2021YFH0060,2021YFH0072,2021ZHFP0045,2021YFN0092);the Fundamental Research Funds for the Central Universities(2018CDLZ-07,2018CDPZH-9,2019CDPZH-23,2020CDLZ-17)。

摘　　要：This study explored the antibiofilm efficacy of 3,3’-diindolylmethane(DIM)on Staphylococcus aureus and its disinfection on common food-contact surfaces.The minimum biofilm inhibitory concentration(MBIC)of DIM on S.aureus was 62.5μmol/L,while it did not impede the bacterial growth evaluated by growth curve and XTT reduction assay.DIM in the concentration range of 31.2-62.5μmol/L demonstrated a dose-dependent antibiofilm activity to S.aureus,as confirmed by light microscopic(LM),confocal laser scanning microscopic(CLSM),and scanning electron microscopic(SEM)analyses.At DIM of62.5μmol/L,the biomass of S.aureus biofilm was significantly reduced by 97%and its average thickness by 58%(P<0.05).DIM of 62.5μmol/L inhibited the bacterial initial adhesion and proliferation,as well as cell motility;the release of extracellular DNA(eDNA)and extracellular polysaccharide(EPS)were reduced by 75%and 69%,respectively.DIM exhibited a strong inhibition to S.aureus biofilm formation on common food-contact surfaces,including 304 stainless steel,glass,and polyvinyl chloride(PVC)but not disperse the mature biofilm.Overall,our investigation identified DIM as a promising antibiofilm agent and its suitability to prevent the biofilm formation of S.aureus on common food-contact surfaces utilized during food processing.

关 键 词：Biofilm inhibition 3 3’-Diindolylmethane Extracellular DNA Extracellular polysaccharide Food-contact surfaces 

分 类 号：TS201.3[轻工技术与工程—食品科学]