Simultaneous determination of ten nucleosides and bases in Ganoderma by micellar electrokinetic chromatography  

作　　者：Feiya Sheng Songsong Wang Xiao Luo Jianbo Xiao Linfeng Hu Peng Li 

机构地区：[1]State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macao,Macao,999078,China [2]School of Basic Medical Sciences,Chengdu University,Chengdu 610106,China [3]Chengdu Institute for Food and Drug Control,Chengdu,610045,China [4]Chongqing Key Laboratory of Medicinal Resources in the Three Gorges Reservoir Region,School of Biological and Chemical Engineering,Chongqing University of Education,Chongqing,400067 China

出　　处：《Food Science and Human Wellness》2022年第2期263-268,共6页食品科学与人类健康（英文）

基　　金：financially supported by the Research Committee of the University of Macao(MYRG2018-00239-ICMS and MYRG2014-00089-ICMS-QRCM);the Macao Science and Technology Development Fund(162/2017/A3);Guangzhou International Science and Technology Cooperation Project(File no.201807010044)。

摘　　要：Ganoderma(lingzhi)is a famous herbal medicine and edible supplement in oriental countries for a long history.In this study,a simple micellar electrokinetic chromatography(MEKC)method was established for the analysis of nucleosides and bases,the major bioactive components in Ganoderma for the first time.By optimizing the borate concentration,the sodium dodecyl sulfate(SDS)concentration and the pH value of running buffer,10 nucleosides and bases achieved an ideal separation.In real sample analysis,the developed method was successfully used to determine the 10 target analytes in 23 batches of Ganoderma samples from different regions.Results indicated that contents of 10 investigated analytes in each sample showed obvious variation.The principal components analysis(PCA)and hierarchical cluster analysis(HCA)analysis classified the samples into three groups,and the HCA tree visualized the relationships which was mainly contributed by geographical partition.The results indicated geographical origin to be an important factor that affect the accumulation of nucleosides and bases in Ganoderma.In summary,this study provides a simple and practical strategy for quality assessment and cultivation reference of Ganoderma.

关 键 词：GANODERMA NUCLEOSIDES BASES Micellar electrokinetic chromatography Quality assessment 

分 类 号：O657.7[理学—分析化学] R284.1[理学—化学]