Runx2缺失对小鼠颞下颌关节髁突软骨细胞肥大分化以及生物力学性能的影响  

The effects of Runx2 gene knockout on the hypertrophic differentiation and biomechanics of condylar chondrocytes in the temporomandibular joint of mice

作　　者：廖立凡 李昱 杨琳 杨欣 LIAO Lifan;LI Yu;YANG Lin;YANG Xin(Key Laboratory of Shaanxi Province for CraniofacialPrecision Medicine Research,Department of Implant Dentistry,College of Stomatology,Xi'an Jiaotong University,China,710004;Urumqi,Department of Oral and Maxillofacial Surgery,General Hospital of Xinjiang Military Region)

机构地区：[1]西安交通大学口腔医院陕西省颅颌面精准医学研究重点实验室,西安交通大学口腔医院种植科,710004 [2]新疆军区总医院口腔颌面外科

出　　处：《实用口腔医学杂志》2022年第3期317-321,共5页Journal of Practical Stomatology

基　　金：西安市创新能力强基计划-医学研究项目(编号:21YXYJ0124);新疆维吾尔自治区自然科学基金面上项目(编号:2021D01C473)。

摘　　要：目的:研究Runt相关转录因子-2(Runx2)缺失对小鼠颞下颌关节(TMJ)髁突软骨细胞的肥大分化及其生物力学性能的影响。方法:创建Runx2^(Agc1CreER)条件性敲除小鼠及其同窝对照小鼠(Cre^(-))模型。采用组织学切片观察、免疫组化染色以及纳米弹性模量检测方法,比较两组小鼠髁突软骨细胞分化及生物力学特性的差异。结果:Runx2敲除导致软骨肥大层丧失以及软骨基质生成减少,髁突软骨中ColX、IHH、PCNA表达显著降低。Runx2^(Agc1CreER)组和Cre^(-)组小鼠髁突软骨纳米弹性模量(MPa)分别为0.0898和0.7620(P<0.05)。结论:Runx2缺失干扰出生后小鼠TMJ髁突软骨细胞肥大分化过程,影响髁突软骨的力学性能。Objective:To investigate of Runx2 gene knockout on the hypertrophic differentiation of chondrocytes and biomechanical properties of the condyle of mice.Methods:Runx2 knockout mice(Runx2^(Agc1CreER))and its littermate control mice(Cre^(-))were used for the study.Histological observation,immunohistochemical staining and nanoindentation test were performed to compare the similarities and differences of the hypertrophic diffentiation and biomechanics between the 2 groups of mice.Results:HE staining observation and immunohistochemical results showed that the lack of Runx2 led to the loss of hypertrophic cartilage layer and reduction of cartilage matrix production in Runx2^(Agc1CreER) mice.The expressions of ColX,IHH and PCNA in the condylar cartilage of Runx2^(Agc1CreER) mice were significantly reduced.The nano-elastic modulus(MPa)of the condylar cartilage of Cre^(-) and Runx2^(Agc1CreER) mice was 0.0898 and 0.7620 respectively(P<0.05).Conclusion:Runx2 is necessary for the hypertrophic differentiation of TMJ condylar chondrocytes in mice after birth.Knockout Runx2 may increase the elastic modulus of condyle cartilage.

关 键 词：Runtx2 软骨细胞 肥大分化 生物力学 颞下颌关节 

分 类 号：R782.6[医药卫生—口腔医学]