生理浓度二苯酮-3暴露对小鼠卵母细胞体外成熟及其质量的影响  

Effects of exposure to benzophenone-3 at physiological concentration on maturation and quality of mouse oocytes in vitro

作　　者：尹锦雯 朱海瑛 肖国宏[3] YIN Jinwen;ZHU Haiying;XIAO Guohong(Department of Obstetricsand Gynecology,Key Laboratory for Major Obstetric Diseases of Guangdong Province,the Third Affiliated Hospital of Guangzhou Medical University,Guangzhou 510150,China;不详)

机构地区：[1]广州医科大学附属第三医院妇产科,广东省产科重大疾病重点实验室,广州510150 [2]广东省妇幼保健院生殖健康与不孕症科,广州511442 [3]广州医科大学附属第二医院妇科,广州510260

出　　处：《实用医学杂志》2022年第9期1051-1055,共5页The Journal of Practical Medicine

基　　金：国家自然科学基金青年科学基金项目(编号:81901483)。

摘　　要：目的研究紫外线吸收剂二苯酮-3(BP-3)在其生理浓度范围内对小鼠卵母细胞体外成熟及其质量的影响,为人类生殖健康的研究提供参考。方法以6~10周龄的ICR雌鼠为研究对象,采用不同浓度的BP-3处理小鼠GV期卵母细胞,观察卵母细胞的体外成熟率。通过检测卵子活性氧(ROS)含量、DNA损伤、早期凋亡和转录水平来探讨BP-3对卵母细胞成熟发育的影响。结果BP-3对卵母细胞成熟的抑制呈剂量依赖性(P<0.05)。与对照组相比,0.8μmol/L BP-3处理提高了卵子中ROS水平,促进了DNA损伤和早期凋亡(P<0.05)。qRT-PCR结果显示,抗氧化基因Gpx1和凋亡相关基因Bcl2的转录水平下调,促凋亡相关基因Bax表达水平提高(P<0.05)。结论在生理浓度范围内的BP-3暴露会降低小鼠卵母细胞的体外成熟水平及其质量。Objective To evaluate the effects of benzophenone-3(BP-3),aultraviolet absorbent,on the maturation and quality of mouse oocytes within the range of physiological concentration,to provides reference for the research of human reproductive health.Methods Female ICR mice aged 6 to 10 weeks were used in this study.GV stage oocytes of mice were treated with different concentrations of BP-3,and the in vitro maturation rate of the oocytes was observed.The effects of BP-3 on the maturation and development of oocytes were investigated by measuring the content of reactive oxygen species(ROS)and detecting DNA damage,theearly apoptosis and transcription levelsof oocytes.Results BP-3 inhibited oocyte maturation in a dose-dependent manner(P<0.05).The ROS level of oocytes treated with 0.8μmol/L BP-3 was higher than the control group,and DNA damage and early death of oocytes were considerably increased(P<0.05).And qRT-PCR results showed that the transcription levels of antioxidant gene Gpxl and apopsis related gene Bcl2 were dounregulated,the expression of pre-apoptotic gene Bax was up-regulated(P<0.05).Conclusion Exposure to physiological concentrations of BP-3 may reduce the rate and quality of mouse oocyte maturation in vitro.

关 键 词：二苯酮-3 卵母细胞 体外成熟 卵子质量 

分 类 号：R394[医药卫生—医学遗传学]