茶树CsbHLH024和CsbHLH133转录因子功能鉴定  

Functional Identification of CsbHLH024 and CsbHLH133 Transcription Factors in Tea Plants

作　　者：刘任坚 王玉源 刘少群[1] 舒灿伟[2] 孙彬妹 郑鹏 LIU Renjian;WANG Yuyuan;LIU Shaoqun;SHU Canwei;SUN Binmei;ZHENG Peng(College of Horticulture,South China Agricultural University,Guangzhou 510642,China;College of Agriculture,South China Agricultural University/Guangdong Province Key Laboratory of Microbial Signals and Disease Control/Integrative Microbiology Research Center,Guangzhou 510642,China)

机构地区：[1]华南农业大学园艺学院,广东广州510642 [2]华南农业大学植物保护学院/广东省微生物信号与作物病害防控重点实验室/群体微生物研究中心,广东广州510642

出　　处：《茶叶科学》2022年第3期347-357,共11页Journal of Tea Science

基　　金：广州市科技计划项目(202102020290);广东省自然科学基金项目(2018A030313089、2021A1515012091)。

摘　　要：茶树叶片毛状体含有多种次生代谢产物,在茶叶外观质量以及茶树响应生物和非生物胁迫方面起着重要作用。通过双荧光分子互补(BiFC)试验、GUS活性染色试验以及过表达试验对茶树叶片毛状体相关候选基因CsbHLH024和CsbHLH133的功能进行鉴定。结果表明,CsbHLH024/CsbHLH133和CsTTG1蛋白在植物中能够相互作用,并且它们的启动子能够在叶片组织中驱动下游基因的表达。进一步将它们分别过表达到野生型拟南芥Col和对应的拟南芥纯合突变体中,发现它们能够影响拟南芥叶片毛状体的形成,恢复突变体的表型,并引起毛状体相关基因表达水平的变化。本研究为进一步揭示茶树叶片毛状体形成的分子调控机制提供理论依据。Tea plant leaf trichomes contain various secondary metabolites and play an important role in the tea appearance quality as well as the response of tea plants to biotic and abiotic stresses.In this study,the function of leaf trichome-related genes CsbHLH024 and CsbHLH133 were analyzed using Bimolecular Fluorescent Complimentary(BiFC),GUS staining and overexpression experiments.The results show that CsbHLH024/CsbHLH133 and CsTTG1 could interact in plants,and their promoters could drive downstream gene expression in leaf tissues.They were further transformed into wild Arabidopsis thaliana(Col)and corresponding homozygous mutants,respectively to get overexpression lines.Both genes could affect the leaf trichome formation in Arabidopsis thaliana,restore the phenotype of the mutants,and induce the expression levels of trichome-related genes.This study provided a theoretical basis for further research on the molecular regulation mechanism of trichome formation in tea leaves.

关 键 词：茶树 毛状体形成 CsbHLH024 CsbHLH133 分子调控 

分 类 号：S571.1[农业科学—茶叶生产加工]