内脂素对超促排卵未孕大鼠子宫内膜容受性的调控作用及机制  

Regulatory effect and mechanism of visfatin on endometrial receptivity of non-pregnant rats with ovarian hyperstimulation

作　　者：李翠[1] 刘静 于倩 易建平[2] LI Cui;LIU Jing;YU Qian(Department of Reproductive Genetics,Tangshan Maternal and Child Health Hospital,Tangshan Hebei 063000,China;Department of Gynecology,Tangshan Maternal and Child Health Hospital,Tangshan Hebei 063000,China)

机构地区：[1]唐山市妇幼保健院生殖遗传科,河北唐山063000 [2]唐山市妇幼保健院妇科,河北唐山063000

出　　处：《临床和实验医学杂志》2022年第9期905-909,共5页Journal of Clinical and Experimental Medicine

基　　金：河北省医学科学研究课题计划(编号:20201475)。

摘　　要：目的研究内脂素对超促排卵未孕大鼠子宫内膜容受性的调控作用及机制。方法32只健康雌性SD大鼠按照随机数字表法分为对照组、模型组、内脂素组、内脂素+L-NAME组,每组各8只。对照组进行0.9%氯化钠溶液腹腔注射,后3组进行超促排卵造模,造模过程中内脂素组给予内脂素腹腔注射,内脂素+L-NAME组给予内脂素及L-NAME腹腔注射。比较各组大鼠妊娠率、胚胎数量、子宫内膜厚度、螺旋动脉数量及整合素αvβ3、同源盒基因10(HOXA10)、白血病抑制因子(LIF)、内皮型一氧化氮合酶(eNOS)、血管内皮生长因子(VEGF)表达水平的差异。结果模型组大鼠的妊娠率、胚胎数量、子宫内膜厚度、螺旋动脉数量及整合素αvβ3、HOXA10、LIF、eNOS、VEGF的表达水平均低于对照组,差异均有统计学意义(P<0.05)。内脂素组大鼠的妊娠率、胚胎数量、子宫内膜厚度、螺旋动脉数量及整合素αvβ3、HOXA10、LIF、eNOS、VEGF的表达水平均高于模型组,差异均有统计学意义(P<0.05)。内脂素+L-NAME组大鼠的妊娠率、胚胎数量、子宫内膜厚度、螺旋动脉数量及整合素αvβ3、HOXA10、LIF、eNOS、VEGF的表达水平均低于内脂素组,差异均有统计学意义(P<0.05)。结论内脂素改善超促排卵未孕大鼠的子宫内膜容受性,激活eNOS/VEGF通路介导的血管新生是内脂素发挥这一改善作用的相关分子机制。Objective To study the regulatory effect and mechanism of visfatin on endometrial receptivity of non-pregnant rats with ovarian hyperstimulation.Methods Thirty-two healthy female SD rats were divided into control group,model group,visfatin group and visfatin+L-NAME group according to random number table,8 rats in each groups.The control group was intraperitoneally injected with normal saline,and the latter three groups were modeled by ovarian hyperstimulation.During the modeling process,visfatin group was intraperitoneally injected with visfatin,visfatin+L-NAME group was intraperitoneally injected with visfatin and L-NAME.The difference of pregnancy rate,embryo number,endometrial thickness,spiral arteries number and the expression level of integrinαvβ3,homeobox gene 10(HOXA10),leukemia inhibitory factor(LIF),endothelial nitric oxide synthase(eNOS)and vascular endothelial growth factor(VEGF)among each groups were compared.Results The pregnancy rate,embryo number,endometrial thickness,spiral arteries number and the expression level of integrinαvβ3,HOXA10,LIF,eNOS and VEGF in the model group were lower than those in the control group,the differences were statistically significant(P<0.05).The pregnancy rate,embryo number,endometrial thickness,spiral arteries number and the expression level of integrinαvβ3,HOXA10,LIF,eNOS and VEGF in the visfatin group were higher than those in the model group,the differences were statistically significant(P<0.05).The pregnancy rate,embryo number,endometrial thickness,spiral arteries number and the expression level of integrinαvβ3,HOXA10,LIF,eNOS and VEGF in the visfatin+L-NAME group lower than those in the visfatin group,the differences were statistically significant(P<0.05).Conclusion Conclusion Visfatin can improve endometrial receptivity of non-pregnant rats with ovarian hyperstimulation,and the activaton of eNOS/VEGF pathway mediated angiogenesis is the related molecular mechanism of visfatin.

关 键 词：大鼠 内脂素 子宫内膜容受性 内皮型一氧化氮合酶 血管内皮生长因子 信号通路 分子机制 

分 类 号：R-332[医药卫生]