基于TCGA数据库分析POSTN在头颈部鳞癌中的表达和临床意义  

Analysis of the expression and clinical significance of POSTN in head and neck squamous carcinoma based on TCGA database

作　　者：张曼 周兰柱[1] 孙哲[1] 吴俊 王文忠[1] ZHANG Man;ZHOU Lan-zhu;SUN Zhe;WU Jun;WANG Wen-zhong(Department of Otorhinolaryngology Head and Neck Surgery,the First Affiliated Hospital of Bengbu Medical College,Bengbu,Anhui 233004,China)

机构地区：[1]蚌埠医学院第一附属医院耳鼻喉头颈外科,安徽蚌埠233004

出　　处：《中华全科医学》2022年第6期1066-1070,共5页Chinese Journal of General Practice

基　　金：安徽省高校自然科学研究项目(KJ2020A0568)。

摘　　要：目的利用在线数据库和生物信息学方法分析POSTN基因在头颈部鳞状细胞癌中的差异表达对其预后的临床意义,寻找头颈部鳞状细胞癌新的生物标志物。方法使用TCGA公共数据库获取头颈部鳞癌患者的基本信息、POSTN的表达情况;利用Kaplan-Meier Plotter分析不同POSTN表达水平的头颈部鳞癌患者生存曲线;采用TIMER数据库分析POSTN与头颈部鳞癌中各种免疫细胞浸润的相关性;GEPIA数据库分析POSTN表达与免疫细胞标志物表达的相关性;GO和KEGG基因富集分析探索POSTN共表达基因的功能及其作用通路。结果POSTN在头颈部鳞癌中高表达(P<0.05),其表达与头颈部鳞癌患者的预后呈正相关;头颈部鳞癌患者的累积生存率与多种免疫细胞的浸润程度相关;POSTN表达水平与头颈部鳞癌中的巨噬细胞浸润程度呈正相关关系(P=0.024),并与M2型巨噬细胞标志物呈正相关关系(P<0.001),POSTN高表达促进巨噬细胞向M2型极化。富集分析显示POSTN与细胞外基质调节过程相关。结论POSTN表达与头颈部鳞癌患者预后及免疫浸润相关,并且其可能通过调节巨噬细胞极化而导致患者的不良预后。Objective To analyse the clinical significance of POSTN differential expression in the diagnosis and prognosis of head and neck squamous cell carcinoma using online databases and bioinformatics methods and to find new biomarkers for this disease.Methods TCGA public database was used to obtain basic information of head and neck squamous carcinoma patients,the expression of POSTN and the correlation between POSTN and clinicopathological parameters of head and neck squamous carcinoma patients.The Kaplan-Meier Plotter was used to analyse the survival curve of head and neck squamous carcinoma patients with different POSTN expression levels.TIMER analysis was used to determine the correlation between POSTN and various immune cell infiltrations in head and neck squamous carcinoma.GEPIA database analysis was used to determine the correlation between POSTN expression and immune cell marker expression.GO and KEGG gene enrichment analysis were used to explore the function of differentially expressed genes and their pathways of action.Results POSTN was highly expressed in head and neck squamous carcinoma(P<0.05),and its expression was positively correlated with prognosis of head and neck squamous carcinoma patients,the cumulative survival rate of head and neck squamous carcinoma patients was correlated with the degree of infiltration of multiple immune cells.POSTN expression level was positively correlated with the degree of macrophage infiltration in head and neck squamous carcinoma(P=0.024)and significantly positively correlated with M2 macrophage markers(P<0.001),with high POSTN expression promoting macrophage polarisation towards M2 type.Enrichment analysis showed that POSTN was associated with extracellular matrix regulatory processes.Conclusion POSTN expression is associated with prognosis and immune infiltration in patients with head and neck squamous carcinoma,and it may contribute to poor patient prognosis by regulating macrophage polarisation.

关 键 词：POSTN基因 头颈部鳞癌 生存分析 免疫细胞浸润 巨噬细胞极化 

分 类 号：R739.91[医药卫生—肿瘤] R730[医药卫生—临床医学]