三元低共熔溶剂高效预处理提高杨木酶水解效率  

The efficient pretreatment with ternary-deep eutectic solvent for facilitating the enzymatic hydrolysis of poplar

作　　者：赵天畅 王杰[1] 侯海旭 李海潮 游婷婷[1] 许凤[1] ZHAO Tianchang;WANG Jie;HOU Haixu;LI Haichao;YOU Tingting;XU Feng(College of Materials Science and Technology,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]北京林业大学材料科学与技术学院,北京100083

出　　处：《林业工程学报》2022年第3期86-92,共7页Journal of Forestry Engineering

基　　金：高等学校学科创新引智计划111项目(B21022);北京市大学生创新创业训练项目(S202010022193);国家自然科学基金(22008010)。

摘　　要：合成两种新型三元低共熔溶剂:氯化胆碱/乙二醇/对羟基苯磺酸和氯化胆碱/1,4-丁二醇/对羟基苯磺酸,分别对杨木进行预处理。采用成分分析、红外光谱、X-射线衍射及扫描电镜等对预处理前后杨木的化学成分、结构及表面形貌的变化规律进行分析,研究低共熔溶剂的氢键供体种类及预处理时间对杨木成分及后续酶水解的影响。成分分析及红外光谱分析结果表明,氯化胆碱/乙二醇/对羟基苯磺酸及氯化胆碱/1,4-丁二醇/对羟基苯磺酸均可在短时间内(0.5~1.5 h)脱除大量木聚糖(>74%)和木质素(>70%),同时保留绝大部分纤维素(>74%)。X-射线衍射仪分析表明,预处理后杨木纤维素晶型仍为纤维素Ⅰ_(β),但结晶度上升。扫描电镜结果显示,样品表面粗糙度增加。酶水解结果表明,延长处理时间及增长氢键供体碳链均可促进纤维素酶水解,24 h酶解效率最高超过95%。因此,氯化胆碱/多元醇/对羟基苯磺酸体系可显著提高杨木的酶水解效率,是一种短时、高效的预处理体系,有望应用于开发低能耗的木质纤维素加工工艺。The targets of minimizing reliance on petroleum and achieving carbon reduction drive the urgent seeking for alternative resources that are sustainable and renewable.Lignocellulosic biomass is a carbon-neutral,renewable,and abundant alternative.The main components of lignocellulosic biomass are cellulose,hemicellulose and lignin glucose obtained through enzymatic hydrolysis of cellulose could be further converted to biofuels and biochemicals.However,the complex matrix of lignocellulosic biomass decreases enzymes accessibility.Pretreatment is necessary to overcome the matrix barrier via removing hemicelluloses and lignin.Various pretreatment methods have been extensively developed,such as acid,alkali,organic solvent,ionic liquid,and deep eutectic solvent(DES) pretreatments.Among the abovementioned pretreatment solvents,DES is considered as an emerging and green solvent,which is cheap,facile,and less toxic.Unfortunately,the developed binary or ternary DES pretreatment cannot effectively shorten time of the enzymatic hydrolysis for high glucose conversion,which significantly reduced the conversion efficiency.In this research,poplar was pretreated by two kinds of ternary deep eutectic solvents(DESs),namely,choline chloride/ethylene glycol/p-hydroxybenzenesulfonic acid and choline chloride/1,4-butanediol/p-hydroxybenzenesulfonic acid.The chemical composition,structure and surface morphology of raw and pretreated poplar samples were analyzed by the composition analysis(NERL),Fourier-transform infrared-spectroscopy(FT-IR),X-ray diffractometer(XRD) and scanning electron microscope(SEM).The effects of pretreatment time and the types of hydrogen bond donors of DESs on the composition of poplar and subsequent enzymatic hydrolysis were studied.The results of NERL and FT-IR showed that both choline chloride/ethylene glycol/p-hydroxybenzenesulfonic acid and choline chloride/1,4-butanediol/p-hydroxybenzenesulfonic acid pretreatments can remove most of xylan(>74%) and lignin(>70%) in a short time(0.5-1.5 h) while preserving most

关 键 词：三元低共熔溶剂 高效预处理 杨木 酶水解 酶解效率 

分 类 号：TQ35[化学工程]