壬基酚对斑马鱼不同组织中抗氧化酶活力的影响  

Effects of Nonylphenol on Activities of Antioxidant Enzymes in Different Tissues of Zebrafish

作　　者：张银杰 张洪昌 凌思源 蒋玲玲 彭程 张卫[1] Zhang Yinjie;Zhang Hongchang;Ling Siyuan;Jiang Lingling;Peng Cheng;Zhang Wei(State Environmental Protection Key Lab of Environmental Risk Assessment and Control on Chemical Processes,School of Re-sources and Environmental Engineering,East China University of Science and Technology,Shanghai 200237,China;State Environmental Protection Key Lab of Environmental Protection and Environmental Health Impact Assessment of Emerging Pollutants,Shanghai Academy of Environmental Sciences,Shanghai 200233,China)

机构地区：[1]国家环境保护化工过程环境风险评价与控制重点实验室,华东理工大学资源与环境工程学院,上海200237 [2]国家环境保护新型污染物环境健康影响评价重点实验室,上海市环境科学研究院,上海200233

出　　处：《生态毒理学报》2022年第2期362-371,共10页Asian Journal of Ecotoxicology

基　　金：上海市科技兴农项目(20190307)。

摘　　要：为研究壬基酚(nonylphenol,NP)对鱼类体内抗氧化酶活力的动态影响,探究NP对鱼类的毒性效应机制,以斑马鱼为受试生物,采用半静态实验法测定NP对斑马鱼的急性半致死浓度(LC_(50)),并以不同浓度(5、50和100μg·L^(-1))的NP进行14 d暴露和7 d净化实验,探究NP对头部、肌肉和内脏团中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽S-转移酶(GST)活力和还原型谷胱甘肽(GSH)含量的影响。结果表明,NP对斑马鱼的24~96 h的LC_(50)为487.6~273.6μg·L^(-1)。NP胁迫下斑马鱼头部SOD活力在5μg·L^(-1)组表现出显著的毒性兴奋效应;肌肉和内脏团的SOD活力在暴露中后期受到诱导,尤其在高浓度胁迫下更快产生该效应。头部和肌肉的CAT活力分别受NP的抑制和诱导作用,但均随时间增加先上升后下降;而CAT在内脏团中的活力受明显抑制后逐渐上升,100μg·L^(-1)组受到的抑制作用更强且更持久。抗氧化酶受NP影响的差异可能与抗氧化酶在各组织内的敏感性和赋存含量密切相关。50μg·L^(-1)和100μg·L^(-1)组内脏团的GST活力随时间延长而增强,并且其平衡水平与NP浓度呈正相关。GSH在头部和肌肉内的含量在初期被NP大量消耗后,逐渐通过生物合成而增加。净化阶段,高浓度NP胁迫后的抗氧化酶活力一般恢复较慢。本研究结果将为NP对水生动物的毒性风险评价提供科学依据。In order to study the dynamic effects of nonylphenol(NP)on the activities of antioxidant enzymes and explore the toxicity of NP to fish,we selected zebrafish(Danio rerio)as the experimental organism,investigated the acute median lethal concentration(LC_(50))of NP via a semi-static toxicity test,and probed the impacts of NP on activities of superoxide dismutase(SOD),catalase(CAT),glutathione S-transferase(GST)and glutathione(GSH)content in zebrafish(head,muscle,and visceral mass)during the 14-d exposure and 7-d purification test at different concentrations(5,50,100μg·L^(-1))of NP.The results showed that the 24-h~96-h LC_(50)values were 487.6~273.6μg·L^(-1).In the head,a significant toxicity effect on SOD activity was found in the 5μg·L^(-1)treatment at the early exposure stage.At the middle and later stages,the SOD activities in muscle and visceral mass were induced espe-cially at a high concentration of NP.Moreover,NP continuously inhibited the CAT activity in head while the activi-ty in muscle was induced at the middle stage.Both of the CAT activities in head and muscle were increased and then decreased over time.As for the visceral mass,the CAT activity was significantly inhibited and then gradually increased,and 100μg·L^(-1)of NP caused more severe and durable inhibition.The effects of NP on the activities of antioxidant enzymes were different due to their varying sensitivities and contents in the three tissues.In the 50μg·L^(-1)and 100μg·L^(-1)of NP-treated groups,the GST activity in visceral mass was increased over time,and the e-quilibrium activity was positively correlated with the NP concentration.After consuming largely by NP at the early stage of exposure,the total GSH contents in head and muscle were increased over time subsequently through bio-synthesis.The enzyme activities basically indicated a slower recovery after exposure to a higher level of NP at the purification stage.Our findings would provide essential information for interpreting the ecological risks of NP in the aquatic ecosyst

关 键 词：壬基酚 斑马鱼 急性毒性 抗氧化酶 敏感指标 

分 类 号：X171.5[环境科学与工程—环境科学]