SD大鼠坐骨神经断裂吻合后行肢体延长模型的建立  

Establishment of a limb-lengthening model after anastomosis of sciatic nerve rupture in SD rats

作　　者：任鹏[1] 刘凯 刘彦士 蔡飞宇 艾合买提江·玉素甫[1] 买买艾力·玉山 Ren Peng;Liu Kai;Liu Yanshi;Cai Feiyu;Yusufu·Aihemaitijiang;Yushan·Maimaiaili(Department of Trauma and Microreconstructive Surgery,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)

机构地区：[1]新疆医科大学第一附属医院骨科中心显微修复外科,乌鲁木齐830054

出　　处：《中华实验外科杂志》2022年第5期983-986,共4页Chinese Journal of Experimental Surgery

基　　金：新疆维吾尔自治区自然科学基金(2020D01C250);国家自然科学基金(81560357)。

摘　　要：目的探讨构建Sprague-Dawley(SD)大鼠坐骨神经断裂吻合后行肢体延长模型。方法运用随机抽样法选取健康成年雄性SD大鼠40只(2组,每组20只),按坐骨神经断裂吻合后行肢体延长手术时间节点定义为8、10周组。先行右侧坐骨神经离断吻合术,待神经愈合到指定时间节点后行股骨延长术。潜伏期5 d,以0.5 mm/d速度延长,牵张期10 d。延长结束后取材,利用大体观察、坐骨神经功能指数(SFI)、神经复合肌肉动作电位(CMAP)及运动神经传导速度(MNCV)、组织学染色和透射电镜技术与对照组比较神经功能恢复的效果。组间比较采用t检验。结果两组实验动物模型建立成功,退出实验计数大鼠5只(12.5%)。10周组SFI(62.39±0.23比49.23±0.16,t=4.871,P<0.001)、CMAP[(38.75±7.23)m/s比(56.31±5.91)m/s,t=6.237,P<0.05]及MNCV[(42.63±7.14)mV比(53.71±4.52)mV,t=2.724,P<0.05]、组织学染色及透射电镜结果显示愈合优于8周组。结论伴随周围神经损伤的牵张成骨过程迫切需要适宜实验动物模型。本研究设计的坐骨神经断裂吻合后行肢体延长的SD大鼠模型成功率高,成本低,短时间内可大批造模。Objective To explore the establishment of a limb lengthening model of Sprague-Dawley(SD)rats after anastomosiss of ciatic nerve rupture.Methods A total of 40 healthy adult male SD rats(2 groups,20 rats in each group)were selected by random sampling,and divided into 8-week group and 10-week group according to the operation schedule of bone lengthening after sciatic nerve anastomosis.The latent stage was 5 days,then the femur was lengthened at a rate of 0.5 mm/d twice a day,and the distraction stage was 10 days.All the rats were then sacrificed,and the recovery of nerve function was compared between the two groups by gross observation,the sciatic nerve function index(SFI),nerve compound muscle action potential(CMAP)and motor nerve conduction velocity(MNCV),histological staining and transmission electron microscopy.T-test was used for comparison.Results The experimental animal models of the two groups were established successfully,and 5 rats(12.5%)were withdrawn from experiment since the death.The results of appearance observation,electromyography[SFI:62.39±0.23 vs.49.23±0.16,t=4.871,P<0.01;CMAP:(38.75±7.23)m/s vs.(56.31±5.91)m/s,t=6.237,P<0.05;MNCV:(42.63±7.14)mV vs.(53.71±4.52)mV,t=2.724,P<0.05],histological staining,and transmission electron microscopy of nerve and bone samples in the 10-week group after limb lengthening were superior to those in the 8-week group.Conclusion Suitable experimental animal models are needed urgently by the distraction osteogenesis process associated with peripheral nerve injury.The SD rat model of limb lengthening after sciatic nerve anastomosis designed in this study has a high success rate.A large number of low-cost models can be made in a short time,which meets the experimental requirements and has clinical and scientific application value.

关 键 词：神经修复 肢体延长 动物模型 

分 类 号：R-332[医药卫生] R688