3株魔芋茎腐病病原菌的分离鉴定与致病性研究  

Isolation and Identification of Three Strains of Pathogen Causing Konjac Stem Rot and Studies on Pathogenicity

作　　者：赵兴丽 贺圣凌 刘思睿 罗林丽 周罗娜 范士杰[1] 周玉锋[2] ZHAO Xingli;HE Shengling;LIU Sirui;LUO Linli;ZHOU Luona;FAN Shijie;ZHOU Yufeng(Institute of Biotechnology,Guizhou Academy of Agricultural Sciences,Guiyang 550006,Guizhou,China;Tea ResearchInstitute,Guizhou Academy of Agricultural Sciences,Guiyang 550006,Guizhou,China)

机构地区：[1]贵州省农业科学院生物技术研究所,贵州贵阳550006 [2]贵州省农业科学院茶叶研究所,贵州贵阳550006

出　　处：《中国蔬菜》2022年第6期56-63,共8页China Vegetables

基　　金：贵州省农业科学院青年科技基金项目[黔农科院青年科技基金(2021)18号];贵州省科技计划项目[黔科合支撑(2021)一般208,黔科合服企(2019)4009,黔科合服企(2021)11号]。

摘　　要：镰刀菌引起的魔芋茎腐病是魔芋栽培过程中重要病害之一,为明确贵州省兴义市魔芋茎腐病的病原菌种类,2019年从贵州省兴义市采集魔芋茎腐病病样,采用组织分离法对病样进行分离,通过形态学鉴定方法结合基于rDNA-ITS与tef序列分析的分子鉴定方法对分离物进行鉴定,并按照柯赫氏法则进行致病性测定。结果显示,经组织分离法共获得3株镰刀菌纯培养物,经鉴定分属于2个种,分别为尖孢镰刀菌(Fusarium oxysporum)和茄腐镰刀菌(F.solani);致病性结果表明,尖孢镰刀菌菌株xymy-8没有致病性,茄腐镰刀菌菌株xymy-7、xymy-9有致病性,且致病性有差异,其中菌株xymy-7的致病性强于菌株xymy-9的致病性。试验结果为魔芋茎腐病防控提供了理论依据。Konjac stem rot caused by Fusarium spp.is one of the important soil-borne diseases during konjac cultivation.In order to clarify the pathogens causing konjac stem rot,this experiment collected several konjac sickened plant samples from Xingyi City,Guizhou Province in 2019;carried out isolation on these samples by tissue separation method;identified the isolates by using morphological identification method combined with molecular method based on rDNA-ITS and tef sequences;then proceeded pathogenicity test according to Koch’s Postulates.The results showed that 3 strains of Fusarium pure culture were obtained through tissue separation method.They were belonged to 2 different species,i.e.,F.oxysporum and F.solani by identification.Further pathogenicity analysis showed that F.oxysporum xymy-8 had no pathogericity,F.solani xymy-7 and xymy-9 were both pathogenic,and their pathogenicity was different.Among them,xymy-7 had stronger pathogenicity than xymy-9.The experimental results had provided theoretical basis for precise prevention and control of konjac stem rot.

关 键 词：魔芋茎腐病 镰刀菌 分子鉴定 致病性 

分 类 号：S436.32[农业科学—农业昆虫与害虫防治]