生防链霉菌PBSH9对马铃薯疮痂病菌的抑制作用及其代谢物培养条件优化  

Inhibitory Effect of Biocontrol Streptomyces sp.Strain PBSH9 on Potato Common Scab and Optimization of Its Metabolites Cultivation Condition

作　　者：王丽玮 万中义 宋亚迪 修志君 于世成 杜美娥 张笑宇[1] WANG Liwei;WAN Zhongyi;SONG Yadi;XIU Zhijun;YU Shicheng;DU Meie;ZHANG Xiaoyu(College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010019,Inner Mongolia,China;Biopesticide Engineering Research Centre of Hubei Province,Wuhan 430070,Hubei,China;College of ErdosEcological Environment Vocational,Erdos 017010,Inner Mongolia,China)

机构地区：[1]内蒙古农业大学园艺与植物保护学院,内蒙古呼和浩特010019 [2]湖北省生物农药工程研究中心,湖北武汉430070 [3]鄂尔多斯生态环境职业学院,内蒙古鄂尔多斯017010

出　　处：《中国蔬菜》2022年第6期64-71,共8页China Vegetables

基　　金：内蒙古科技计划项目(2019GG180);内蒙古科技成果转化项目(2019CG026);“草原英才”工程内蒙古自治区产业创新人才团队项目;2021年内蒙古自治区级大学生创新创业训练计划项目(202110129013)。

摘　　要：采用平板对峙法探究了生防链霉菌PBSH9对马铃薯疮痂病菌加利利链霉菌PS1的抑菌活性,并采用单因素试验优化菌株PBSH9的培养条件。结果表明,PBSH9对PS1有较强的抑制作用,传代培养3代,对PS1的抑制作用没有减弱,抑制率均大于70%;PBSH9代谢物也可抑制PS1的生长,抑制率为75.21%;PBSH9及其代谢物都可使PS1菌丝之间发生交联、断裂,气生菌丝变短、由弯变直、膨胀变粗,孢子变形,产孢量下降。PBSH9的培养条件优化试验结果表明,每100 mL液体高氏1号培养基中接入7 mL PBSH9的种子液,pH为7,28℃下恒温培养9 d时,PBSH9代谢物抑菌效果最好,抑菌圈直径为23.67 mm。This study explored the inhibitory effects of biocontrol Streptomyces sp.PBSH9 on potato common scab caused by Streptomyces galilaeus(PS1)by plate confrontation method,and optimized the cultivation conditions of strain PBSH9 by using single factor experiment.The results indicated that PBSH9 had stronger inhibiting effect on PS1.After 3 generations’subculture,its inhibiting effects on PS1 were not weakened.And the inhibition ratios were all over 70%.PBSH9 metabolites could also inhibit PS1 growth,and the inhibition rate was 75.21%.PBSH9 and PBSH9 metabolites could lead to cross-linking between hypha,fracture,aerial hypha shorten,bending to straightened,inflation becoming thicker,spore being out of shape,sporulation quantity going down.The results of PBSH9 cultivation condition optimization experiment indicated that put in 7 mL seed solution of biocontrol Streptomyces sp.PBSH9 in each 100 mL of liquid Gao’s No.1 medium,with 7 pH value,and under 28℃ constant temperature cultivation for 9 days,the inhibiting effect of PBSH9 metabolite was the best.The dimeter of inhibitory zone was 23.67 mm.

关 键 词：马铃薯 生防链霉菌 马铃薯疮痂病 培养条件 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]