结直肠癌组织miR-485-5p,miR-1207-3p和miR-590-3p水平表达与临床病理特征及生存的相关性研究  

Correlation of miR-485-5p,miR-1207-3p,miR-590-3p Level Expression and Clinicopathologic Characteristics and Survival in Colorectal Cancer Tissue

作　　者：马胜辉[1] 崔雪琴 李建华[1] 王翔[1] MA Sheng-hui;CUI Xue-qin;LI Jian-hua;WANG Xiang(Department of Oncology,Central Hospital of Chengde City,Hebei Chengde 067000,China;Department of Integrative Traditional Chinese and Western Medicine,Hebei Geriatric Hospital,Shijiazhuang 050011,China)

机构地区：[1]承德市中心医院肿瘤科,河北承德067000 [2]河北省老年病医院中西医结合科,石家庄050011

出　　处：《现代检验医学杂志》2022年第3期16-20,32,共6页Journal of Modern Laboratory Medicine

基　　金：河北省医学科学研究课题(20211022),资助单位:河北省卫生健康委员会。

摘　　要：目的研究miR-485-5p,miR-1207-3p和miR-590-3p在结直肠癌演进过程中的表达变化。方法选取2019年1月~2020年1月承德市中心医院病理科归档资料齐全完整的84例结直肠癌组织石蜡标本和距离癌组织边缘5cm的癌旁组织石蜡标本。采用免疫组织化学法及荧光定量PCR法检测以直肠组织制作的标本miR-485-5p,miR-1207-3p和miR-590-3p,分析miR-485-5p,miR-1207-3p和miR-590-3p在结直肠癌组织演进过程中的表达变化。结果癌组织miR-485-5p(3.25±0.32 fmol/L),miR-1207-3p(1.45±0.14 fmol/L)和miR-590-3p(2.63±0.26 fmol/L)表达均低于癌旁组织(7.65±0.76 fmol/L,5.81±0.58 fmol/L,8.95±0.89 fmol/L),差异有统计学意义(t=48.900,66.970,62.470,均P<0.05)。Ⅲ~Ⅳ期、中、低分化、有淋巴结转移、有肝转移患者miR-485-5p,miR-1207-3p和miR-590-3p表达分别低于Ⅰ~Ⅱ期、高分化、无淋巴结转移、无肝转移患者,差异有统计学意义(t/F=21.338~52.835,均P<0.05)。死亡组患者miR-485-5p(1.52±0.15 fmol/L),miR-1207-3p(0.50±0.05 fmol/L)和miR-590-3p(1.15±0.11 fmol/L)均低于存活组(4.98±0.49 fmol/L,2.40±0.24 fmol/L,4.11±0.41 fmol/L),差异具有统计学意义(t=27.820,31.370,28.520,均P<0.05)。ROC曲线分析显示,三项联合曲线下面积、敏感度和特异度分别为0.823,86.30%和87.32%;miR-485-5p分别为0.699,85.78%和86.41%;miR-1207-3p分别为0.768,85.59%和71.14%;miR-590-3p分别为0.719,83.62%和87.62%,三项联合诊断价值大于单独检测。miR-485-5p,miR-1207-3p和miR-590-3p之间相关性分析显示,miR-485-5p与miR-1207-3p呈正相关(r=0.457,P=0.005);miR-485-5p与miR-590-3p呈正相关(r=0.314,P=0.003);miR-1207-3p与miR-590-3p呈正相关(r=0.332,P=0.002)。结论血清miR-485-5p,miR-1207-3p和miR-590-3p水平联合检测对诊断结直肠癌、判断恶化程度有一定临床价值,且可能作为结直肠癌患者潜在的预后标志物。Objective To investigate the expression changes of miR-485-5p,miR-1207-3p and miR-590-3p during the progression of colorectal cancer.Methods The archive information which was complete full of 84 cases of colorectal cancer tissue paraffin specimens and 5cm of cancerous tissue edge tissue adjacent to carcinoma specimens of paraffin was selected in Department of Pathology,Central Hospital of Chengde City from January 2019 to January 2020.Used the immunohistochemical method and fluorescence quantitative PCR method to detect miR-485-5p,miR-1207-3p,miR-590-3p,analysis of miR-485-5p,miR-1207-3p and miR-590-3p in the expression changes in the process of evolution of colorectal cancer in the organization.Results The expressions of miR-485-5p(3.25±0.32 fmol/L),miR-1207-3p(1.45±0.14 fmol/L)and miR-590-3p(2.63±0.26 fmol/L)in cancer tissues were all lower than in the tissues adjacent to the cancerous tissue(7.65±0.76 fmol/L,5.81±0.58 fmol/L,8.95±0.89 fmol/L),the differences were statistically significant(t=48.900,66.970,62.470,all P<0.05).The expressions of miR-485-5p,miR-1207-3p and miR-590-3p in patients with stageⅢ~Ⅳ,moderately and poorly differentiated,lymph node metastasis and liver metastasis were lower than those of stageⅠ~Ⅱ,well differentiated,no lymph node metastasis and the patients without liver metastasis,the differences were statistically significant(t/F=21.338~52.835,all P<0.05).The miR-485-5p(1.52±0.15 fmol/L),miR-1207-3p(0.50±0.05 fmol/L)and miR-590-3p(1.15±0.11 fmol/L)of the death group were lower than those of the survival group(4.98±0.49 fmol/L,2.40±0.24 fmol/L,4.11±0.41 fmol/L),and the differences were statistically significant(t=27.820,31.370,28.520,all P<0.05).ROC curve analysis showed that the area under the three joint curves,sensitivity and specificity were 0.823,86.30%and 87.32%respectively.MiR-485-5p was 0.699,85.78%and 86.41%respectively.MiR-1207-3p was 0.768,85.59%and 71.14%respectively.MiR-590-3p were 0.719,83.62%and 87.62%respectively.Three associations had high predictive va

关 键 词：结直肠癌 微小核糖核酸-485-5p 微小核糖核酸-1207-3p 微小核糖核酸-590-3p 

分 类 号：R735.3[医药卫生—肿瘤] R730.43[医药卫生—临床医学]