非小细胞肺癌患者血浆miR-520f,miR-143-3p表达与病理特征和预后的相关性研究  

Correlation Study on Plasma miR-520f and miR-143-3p Expression with Pathological Features and Prognosis in Patients with Non-small Cell Lung Cancer

作　　者：张玲玲 张小霞[1] 於潇潇 葛琴[1] ZHANG Ling-ling;ZHANG Xiao-xia;YU Xiao-xiao;GE Qin(Department of Laboratory Medicine,Nantong Tumor Hospital,Jiangsu Nantong 226361,China)

机构地区：[1]南通市肿瘤医院检验科,江苏南通226361

出　　处：《现代检验医学杂志》2022年第3期69-72,78,共5页Journal of Modern Laboratory Medicine

摘　　要：目的探讨血浆微小核糖核酸(micro RNA,miRNA)-520f和miR-143-3p表达与非小细胞肺癌(non-small cell lung cancer,NSCLC)患者病理特征和预后的关系。方法选择2015年1月~2017年1月南通市肿瘤医院收治的81例首次确诊NSCLC患者(NSCLC组)和62例体检正常者(对照组)。均采集外周血浆,采用实时荧光定量PCR(qRT-PCR)检测miR-520f和miR-143-3p表达,分析miR-520f和miR-143-3p表达与NSCLC临床病理特征的关系。以随访期间肿瘤复发、远处转移、死亡为终点事件,Kaplan-Meier和COX回归分析miR-520f和miR-143-3p表达与NSCLC患者预后的关系。结果NSCLC组血浆miR-520f(1.82±0.32),miR-143-3p(0.35±0.06)表达低于对照组(2.51±0.49,0.94±0.11),差异均有统计学意义(t=10.160,40.986,均P<0.05)。miR-520f表达与NSCLC患者TNM分期、淋巴结转移有关(t=4.335,4.983,均P<0.05),miR-143-3p表达与分化程度、TNM分期和淋巴转移有关(t=5.035,3.349,7.604,均P<0.05),差异均有统计学意义。Kaplan-Meier生存分析结果显示低miR-520f(58.97%,76.92%)和miR-143-3p(58.33%,75.00%)表达,NSCLC患者无疾病进展(progression-free survival,PFS)与总体生存(overall survival,OS)生存率低于高miR-520f表达(90.48%,95.24%)和高miR-143-3p表达(88.89%,95.56%),差异均有统计学意义(Log Rankχ^(2)=9.270,5.303,P=0.02,0.021;Log Rnakχ^(2)=9.079,6.857,P=0.003,0.009)。淋巴结转移(OR=1.680,95%CI:1.423~1.956),miR-520f(OR=1.836,95%CI:1.658~2.358),miR-143-3p(OR=2.169,95%CI:2.015~3.959)与NSCLC不良预后有关(均P<0.05)。结论miR-520f和miR-143-3p在NSCLC患者中表达降低,且与NSCLC细胞恶性增殖和侵袭行为以及NSCLC患者不良预后有关。Objective To investigate the relationship between the expression of plasma miR-520f and miR-143-3p and the pathological features and prognosis of non-small cell lung cancer(NSCLC)patients.Methods From January 2015 to January 2017,81 patients with NSCLC diagnosed for the first time(NSCLC group)and 62 normal subjects(control group)were selected from Nantong Tumor Hospital.Peripheral blood was collected,and the expressions of miR-520f and miR-143-3p were detected by real-time fluorescence quantitative PCR(qRT-PCR),and the relationship between the expressions of miR-520f and miR-143-3p and the clinicopathological characteristics of NSCLC was analyzed.With tumor recurrence,distant metastasis,and death as endpoint events during follow-up,Kaplan-Meier and COX regression analysis were conducted to analyze the relationship between miR-520f and miR-143-3p expression and the prognosis of NSCLC patients.Results The expression of plasma miR-520f(1.82±0.32)and miR-143-3p(0.35±0.06)was lower than that in the control group(2.51±0.49,0.94±0.11),the differences were statistically significant(t=10.160,40.986,all P<0.05).The expression of miR-520f was associated with the TNM stage and lymph node metastasis of NSCLC patients(t=4.335,4.983,all P<0.05),and the expression of miR-143-3p was associated with the degree of differentiation,TNM stage and lymph node metastasis(t=5.035,3.349,7.604,all P<0.05),the differences were statistically significant,respectively.Kaplan-Meier survival analysis showed that the PFS and OS survival rates of NSCLC patients with low miR-520f(58.97%,76.92%)and miR-143-3p(58.33%,75.00%)expression were lower than those with high miR-520f(90.48%,95.24%)and miR-143-3p expression(88.89%,95.56%),the differences were statistically significant(Log Rankχ^(2)=9.270,5.303,P=0.002,0.021;Log Rnakχ^(2)=9.079,6.857,P=0.003,0.009).Multivariate COX regression analysis showed that lymphatic metastasis(OR=1.680,95%CI:1.423~1.956),low expression of miR-520f(OR=1.836,95%CI:1.658~2.358)and miR-143-3p(OR=2.169,95%CI:2.015~3.959)

关 键 词：非小细胞肺癌 微小核糖核酸 

分 类 号：R734.2[医药卫生—肿瘤] R730.43[医药卫生—临床医学]