非小细胞肺癌组织中miR-30a和CD73的表达水平与预后相关性分析  

Correlation Analysis of Tissue miR-30a and CD73 Expression Levels and Prognosis in Non-small Cell Lung Cancer

作　　者：马彦娥[1] 苏虎艳 王倩如 郝光军[1] MA Yan-e;SU Hu-yan;WANG Qian-ru;HAO Guang-jun(Center of Cancer Diagnosis and Treatment,the First Hospital of Yulin City,Shaanxi Yulin 719000,China)

机构地区：[1]榆林市第一医院肿瘤诊疗中心,陕西榆林719000

出　　处：《现代检验医学杂志》2022年第3期73-78,共6页Journal of Modern Laboratory Medicine

基　　金：榆林市科技计划项目(项目编号【2019】185号-41):miR-376a调控TRIM22-CDC14A参与小细胞肺癌顺铂耐药性的研究。

摘　　要：目的探究微小核糖核酸(micro RNA,miR)-30a,胞外-5’核苷酸酶(ecto-5’-nucleotidase,CD73)在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中的表达及其预后相关性。方法选取2015年8月~2019年12月于榆林市第一医院术后病理检查证实为NSCLC患者癌组织120例为研究组,50例正常癌旁组织(距离癌组织5cm以上)为对照组,采用实时荧光定量PCR(qRT-PCR)技术检测组织标本中miR-30a相对表达量,免疫组织化学法检测CD73的表达情况,分析miR-30a,CD73表达水平与NSCLC患者临床病理参数及生存时间之间的关系,多因素COX回归分析影响NSCLC患者预后的危险因素。结果miR-30a在NSCLC癌组织中的表达水平(1.17±0.21)明显低于正常癌旁组织(1.62±0.48),差异有统计学意义(t=8.521,P=0.000);CD73在NSCLC癌组织及癌旁正常组织中的阳性表达率分别为63.33%(76/120)和32.00%(16/50),差异有统计学意义(χ^(2)=13.955,P=0.000);miR-30a高表达组NSCLC患者一年累积生存率(86.89%)明显高于低表达组(59.32%)(Log Rankχ^(2)=11.652,P=0.000);CD73高表达组NSCLC患者一年累积生存率(58.82%)明显低于低表达组(92.31%)(Log Rankχ^(2)=16.894,P=0.000);多因素COX回归分析,结果显示分化程度低(HR=1.677,95%CI:1.092~2.576),发生淋巴结转移(HR=1.574,95%CI:1.043~2.376),miR-30a低表达(HR=1.479,95%CI:1.027~2.130),CD73高表达(HR=1.501,95%CI:1.059~2.128)是影响NSCLC患者预后不良的危险因素。结论NSCLC癌组织中,miR-30a表达下调,CD73表达上调,二者水平异常表达可能与NSCLC的发生发展有关,低miR-30a和高CD73与患者预后不良有关。Objective To investigate the expression of microRNA(miR)-30a and ecto-5’-nucleotidase(CD73)in non-small cell lung cancer(NSCLC)tissues and their prognostic correlation.Methods From August 2015 to December 2019,120 cases of cancerous tissues from NSCLC patients confirmed by postoperative pathological examination in the First Hospital of Yulin City were selected as the study group,and 50 cases of normal adjacent tissues(more than 5cm away from cancer tissue)were selected as the control group.The relative expression of miR-30a in tissue samples was detected with quantitative real-time PCR(qRTPCR)technology,the expression of CD73 was detected with immunohistochemical method,the relationship between the expression levels of miR-30a and CD73 and the clinicopathological parameters and survival time of NSCLC patients was analyzed,the risk factors affecting the prognosis of NSCLC patients were analyzed with multivariate COX regression.Results The expression level of miR-30a in NSCLC cancer tissues(1.17±0.21)was significantly lower than that of normal adjacent tissues(1.62±0.48),and the difference was statistically significant(t=8.521,P=0.000).The positive expression rate of CD73 in NSCLC cancer tissues and normal adjacent tissues was 63.33%(76/120)and 32.00%(16/50),respectively,and the difference was statistically significant(χ^(2)=13.955,P=0.000).The one-year cumulative survival rate of NSCLC patients in the miR-30a high expression group(86.89%)was significantly higher than that in the low expression group(59.32%)(Log Rankχ^(2)=11.652,P=0.000).The one-year cumulative survival rate of NSCLC patients in the CD73 high expression group(58.82%)was significantly lower than that in the low expression group(92.31%)(Log Rankχ^(2)=16.894,P=0.000).Multivariate COX regression analysis showed low differentiation(HR=1.677,95%CI:1.092~2.576),lymph node metastasis(HR=1.574,95%CI:1.043~2.376),and low miR-30a expression(HR=1.479,95%CI:1.027~2.130)and high CD73 expression(HR=1.501,95%CI:1.059~2.128)were risk factors affecting the po

关 键 词：非小细胞肺癌 微小核糖核酸-30a 胞外5’-核苷酸酶 生存分析 

分 类 号：R734.2[医药卫生—肿瘤] R730.43[医药卫生—临床医学]