IL-12A和EBI3在OSCC中的表达及其与免疫微环境的关系  

Expression of IL-12A and EBI3 in Oral Squamous Cell Carcinoma and Their Effect on Immune Microenvironment

作　　者：吕宜楠 胡雅英 郑小风 杨可 张佳莉 LV Yinan;HU Yaying;ZHENG Xiaofeng;YANG Ke;ZHANG Jiali(Department of Oral Histopathology,Hospital of Stomatology,Wuhan University,Wuhan 430079,China;The State Key Laboratory Breeding Base of Basic Science of Stomatology(Hubei_MOST)&Key Laboratory of Oral Biomedicine Ministry of Education,School of Stomatology,Wuhan University,Wuhan 430079,China)

机构地区：[1]武汉大学口腔医院口腔病理科,湖北武汉430079 [2]口腔基础医学省部共建国家重点实验室培育基地和口腔生物医学教育部重点实验室,武汉大学口腔医学院,湖北武汉430079

出　　处：《口腔医学研究》2022年第6期533-539,共7页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:81972552)。

摘　　要：目的:探讨IL-35两个亚基IL-12A和EBI3在口腔鳞状细胞癌(OSCC)组织中的表达,及其与OSCC患者临床病理特征、预后和免疫微环境的关系。方法:通过组织芯片和免疫组织化学法检测186例OSCC患者的正常组织及癌组织中的IL-35亚基IL-12A和EBI3的表达水平,中性粒细胞和CD4+T细胞的浸润水平;通过TCGA数据库和TIMER数据库分析IL-12A和EBI3与OSCC无病间期和免疫细胞浸润的关系。结果:IL-12A和EBI3在肿瘤组织中表达显著高于正常上皮(P<0.001)。IL-12A和EBI3在肿瘤组织中的表达具有相关性(r=0.45,P<0.01),IL-12A和EBI3的表达水平只与OSCC的病理分级有关。IL-12A和EBI3低表达患者的预后更佳(P<0.05),无病间期也更长(P<0.05)。免疫浸润分析发现EBI3和中性粒细胞浸润相关(r=0.311,P<0.0001),IL12A与CD4+T细胞有关(r=0.254,P<0.05)。结论:IL-35亚基IL-12A和EBI3在OSCC中表达上升,影响OSCC患者的预后并影响免疫相关细胞浸润水平。Objective:To investigate the expression of IL-35 subunits IL12A and EBI3 in oral squamous cell carcinoma(OSCC)and theirs relationship with clinicopathological features,prognosis,and immune microenvironment.Methods:The expression levels of IL-35 subunits IL-12A and EBI3,and neutrophil and CD4T cell infiltration in normal and cancer tissues of 186 OSCC patients were detected by tissue microarray and immunohistochemistry.TCGA database and TIMER database were used to analyze the relationship between IL-12A and EBI3 and disease-free interval time and immune cell infiltration of OSCC.Results:The expression of IL-12A and EBI3 in tumor tissue was significantly higher than that in normal epithelium(P<0.001).The expression levels of IL-12A and EBI3 were correlated in tumor tissues(r=0.45,P<0.01).Kaplan-Meier survival analysis showed that patients with low expression of IL-12A and EBI3 had better prognosis(P<0.05)and longer disease-free interval time(P<0.05).IL-12A was associated with CD4T infiltration(r=0.254,P<0.05).EBI3 was associated with neutropil infiltration(r=0.311,P<0.0001),respectively.Conclusion:The increased expression of IL-35 subunits IL-12A and EBI3 in OSCC can affect the prognosis of OSCC patients and the level of immune related cell infiltration.

关 键 词：白细胞介素-35 口腔鳞状细胞癌 预后 肿瘤微环境 

分 类 号：R73[医药卫生—肿瘤]