聚腺嘌呤保护的金纳米团簇与金属有机框架材料ZIF-8复合物的聚集诱导发光增强及用于抗坏血酸的高灵敏荧光检测  

Polyadenine-capped Gold Nanoclusters Incorporated into Zeolitic Imidazolate Framework-8 with Aggregation-induced Fluorescence Emission Enhancement for High-Sensitivity Fluorescence Detection of Ascorbic Acid

作　　者：曹程程 林祥芳 任晨宇 苏磊[1] CAO Cheng-Cheng;LIN Xiang-Fang;REN Chen-Yu;SU Lei(School of Chemistry and Biological Engineering,University of Science and Technology Beijing,Beijing 100083,China)

机构地区：[1]北京科技大学化学与生物工程学院,北京100083

出　　处：《分析化学》2022年第6期932-939,共8页Chinese Journal of Analytical Chemistry

摘　　要：纳米金属有机框架材料(NMOFs)在生物诊断和治疗领域中具有良好的应用前景。本研究建立了一种基于聚腺嘌呤保护的金纳米团簇(PolyA-AuNC)和金属有机框架沸石咪唑酯骨架-8(ZIF-8)纳米复合材料的荧光分析方法,用于血清中抗坏血酸(AA)的高灵敏检测。所制备的纳米复合材料(Poly A-Au NCs@ZIF-8)通过MOF(即ZIF-8)的限制效应显示出聚集诱导的荧光发射增强现象。当AA与ZIF-8作用时,复合材料的骨架被破坏,PolyA-AuNCs从聚集态转变为分散态,导致荧光强度大大降低。将本方法用于AA的检测,线性范围为10~100μmol/L,检出限(LOD, S/N=3)为0.3μmol/L。本方法可在2 min内完成AA的检测,对AA的选择性高。将本方法用于检测人血清中的AA,回收率为95.7%~100.9%。Nano metal organic frameworks(NMOFs)have been widely used in biological diagnosis and treatment systems. In this work, a fluorescence analysis method based on gold nanoclusters(AuNCs)and metal organic framework zeolitic imidazolate framework-8(ZIF-8)composite materials was established for high-sensitivity detection of ascorbic acid(AA). The obtained nanocomposite material(AuNCs@ZIF-8)through the confinement effect of MOF(ZIF-8)showed aggregation-induced emission(AIE)enhancement.When AA was combined with ZIF-8, the framework of the composite material was destroyed, and the fluorescence intensity was greatly reduced due to the transition of AuNCs from the aggregate state to the dispersed state. The linear range of AA detection was measured to be 10–100 μmol/L, and the limit of detection(LOD, S/N=3)was 0.3 μmol/L This method could complete the detection of AA within 2 minutes,showing high selectivity for AA detection. This method was used to detect AA in human serum with good results, and the recoveries were 95.7% –100.9%.

关 键 词：沸石咪唑酯骨架-8 金纳米团簇 荧光检测 抗坏血酸 

分 类 号：O657.3[理学—分析化学] R446.1[理学—化学]