苦瓜杂交一代种子纯度InDel分子标记鉴定技术研究  

Study on InDel Molecular Marker Identification Technology for Purity of First-generation Hybrid Seeds of Bitter Gourd(Momordica charantia)L.

作　　者：袁凡崇 陈木溪 陈如珠 陈银霞 胡开林[1] 程蛟文[1] 郑汉藩 YUAN Fanchong;CHEN Muxi;CHEN Ruzhu;CHEN Yinxia;HU Kailin;CHENG Jiaowen;ZHENG Hanfan(College of Horticulture,South China Agricultural University,Guangzhou Guangdong 510642,China;Guangdong Helinong Seeds Industry Co.,Ltd.,Shantou Guangdong 515800,China;Guangdong Helinong Agricultural Research Institute Co.,Ltd.,Shantou Guangdong 515800,China)

机构地区：[1]华南农业大学园艺学院,广东广州510642 [2]广东和利农种业股份有限公司,广东汕头515800 [3]广东和利农农业研究院有限公司,广东汕头515800

出　　处：《种子》2022年第5期5-9,共5页Seed

基　　金：广东省科技专项资金“大专项+任务清单”(190826095564951);广州市科技计划项目(202102020800)。

摘　　要：以6份苦瓜自交系及其配组的3个苦瓜杂交一代组合为试验材料,对6份苦瓜自交系进行高深度重测序,鉴定获得覆盖苦瓜全基因组的InDel变异位点信息,并针对InDel变异位点设计引物进行PCR扩增验证,成功开发3个苦瓜杂交一代组合的各自特异多态性InDel分子标记,将多态InDel分子标记应用于苦瓜杂交一代种子纯度鉴定。结果表明,InDel分子标记纯度鉴定结果与田间种植鉴定结果一致,初步建立了苦瓜杂交一代种子纯度InDel分子标记鉴定技术体系。In this study,six bitter gourd inbred lines and three bitter gourd hybrids one generation were used as experimental materials.First,the six bitter gourd inbred lines were re-sequenced in a high-depth to identify the InDel(insertion and deletion)variants covering the whole genome of bitter gourd.Then,primers were designed for PCR amplification of InDel variant sites and the respective specific polymorphic InDel molecular markers for the three bitter gourd hybrids one generation were successfully developed.Finally,the polymorphic InDel molecular markers were applied to the seed purity identification of bitter gourd hybrid.The results of the InDel molecular marker purity identification were consistent with that of the field planting identification method.Thus,the InDel molecular marker identification technology system for the purity of bitter gourd hybrid seeds was initially established.

关 键 词：苦瓜 杂交一代种子 INDEL 分子标记 纯度鉴定 

分 类 号：S642.5[农业科学—蔬菜学]