HOXA10基因敲低对非小细胞肺癌安罗替尼敏感性的影响  

Effect of HOXA10 knockdown on the sensitivity of non-small cell lung cancer cells to anlotinib

作　　者：李俞羲 路遥 田野[2] LI Yuxi;LU Yao;TIAN Ye(Department of Anesthesiology,The Fourth Affiliated Hospital of China Medical University,Shenyang 110032,China;Department of Thoracic Surgery,The Fourth Affiliated Hospital of China Medical University,Shenyang 110032,China)

机构地区：[1]中国医科大学附属第四医院麻醉科,沈阳110032 [2]中国医科大学附属第四医院胸外科,沈阳110032

出　　处：《中国医科大学学报》2022年第6期508-512,517,共6页Journal of China Medical University

基　　金：辽宁省自然科学基金(JCZR2020016)。

摘　　要：目的 探讨同源异型盒基因A10 (HOXA10)对非小细胞肺癌(NSCLC)细胞安罗替尼敏感性的影响。方法 采用实时定量PCR检测HOXA10在NSCLC细胞中的表达。应用小干扰RNA在A549和PC-9细胞中敲低HOXA10表达,并用不同浓度安罗替尼处理细胞。Western blotting检测HOXA10蛋白表达;CCK-8法检测细胞增殖活性,以明确安罗替尼的细胞毒性,并计算半数抑制浓度(IC_(50));克隆形成实验检测细胞增殖;Transwell实验检测细胞迁移和侵袭能力;流式细胞术检测细胞凋亡。结果 HOXA10在NSCLC细胞中呈高表达;敲低HOXA10能够降低安罗替尼在A549和PC-9细胞中的IC_(50);敲低HOXA10能够增强安罗替尼对肿瘤细胞增殖、迁移和侵袭的抑制能力;敲低HOXA10能够促进安罗替尼诱导的细胞凋亡。结论 敲低HOXA10能够增加NSCLC细胞对安罗替尼的敏感性。Objective To investigate the effect of homeobox A10(HOXA10) on the sensitivity of non-small cell lung cancer(NSCLC) cell lines to anlotinib. Methods Quantitative real-time PCR was used to determine the expression of HOXA10 mRNA in NSCLC cells.A549 and PC-9 cells were transfected with si-HOXA10,and the expression of HOXA10 protein was detected using Western blotting.The transfected cells were then treated with anlotinib. Cell viability and half-maximal inhibitory concentration(IC_(50)) were determined using the CCK-8 assay. Colony-forming ability was assessed using a colony formation assay. Cell migration and invasion were determined using Transwell assay. Annexin-V/PI staining and flow cytometry were performed to detect cell apoptosis. Results HOXA10 was remarkably upregulated in NSCLC cells. HOXA10 knockdown significantly decreased the IC_(50) values of anlotinib in both A549 and PC-9 cells.HOXA10 knockdown enhanced the inhibitory effect of anlotinib on colony formation,cell migration,and cell invasion. HOXA10 knockdown promoted anlotinib-induced apoptosis. Conclusion HOXA10 knockdown increases the sensitivity of NSCLC cells to anlotinib.

关 键 词：同源异型盒基因A10 安罗替尼 非小细胞肺癌 敏感性 

分 类 号：R734[医药卫生—肿瘤]