胆管癌组织中HMGB1和MMP-9的表达情况及与临床病理特征、预后的关系  

Expression of HMGB1 and MMP-9 in Cholangiocarcinoma and their Relationship with Clinicopathological Features and Prognosis

作　　者：尹治清 陆晓[1] YIN Zhi-qing;LU Xiao(Department of Interventional,Anyang Cancer Hospital,Anyang 433000,Henan Province,China)

机构地区：[1]安阳市肿瘤医院介入科,河南安阳433000

出　　处：《罕少疾病杂志》2022年第7期65-67,共3页Journal of Rare and Uncommon Diseases

基　　金：河南省医学科技攻关计划项目(LHGJ20191290)。

摘　　要：目的探讨胆管癌组织中高迁移率族蛋白1(HMGB1)和金属基质蛋白酶-9(MMP-9)的表达情况及与临床病理特征、预后的关系。方法选取75例胆管癌患者,采集所有患者术中切除病理组织标本75例,另采集正常癌旁组织进行对照。免疫组化染色观察各组织标本中HMGB1和MMP-9蛋白表达情况,分析胆管癌组织中HMGB1、MMP-9与临床病理特征、预后的关系。结果胆管癌组织中HMGB1、MMP-9蛋白阳性表达率分别为64.00%、72.00%,显著高于癌旁正常组织17.33%、13.33%(P<0.05)。肝癌组织中HMGB1、MMP-9蛋白阳性表达呈正相关(P<0.05)。有门静脉浸润、淋巴结转移及低分化程度的患者HMGB1、MMP-9蛋白阳性表达率高于无门静脉浸润、无淋巴结转移及中高分化程度的患者(P<0.05)。与HMGB1阴性表达比较,阳性表达患者生存率均显著降低(P<0.05)。与MMP-9阴性表达比较,阳性表达患者生存率均显著降低(P<0.05)。结论原发性胆管癌组织中存在HMGB1和MMP-9异常高表达,与患者不良预后有关,在疾病的发生及进展过程中起协同作用。Objective To investigate the expression of high mobility group box 1(HMGB1)and metalloproteinase-9(MMP-9)in cholangiocarcinoma tissues and their relationship with clinicopathological features and prognosis.Methods A total of 75 patients with cholangiocarcinoma were selected,and 75 cases of intraoperative pathological tissue samples were collected from all patients,and normal adjacent tissues were collected for control.Immunohistochemical staining was used to observe the protein expression of HMGB1 and MMP-9 in each tissue specimen,and the relationship between HMGB1 and MMP-9 in cholangiocarcinoma tissue with clinicopathological characteristics and prognosis was analyzed.Results The positive expression rates of HMGB1 and MMP-9 proteins in cholangiocarcinoma tissues were 64.00% and 72.00%,which were higher than adjacent normal tissues 17.33% and 13.33%(P<0.05).The positive expressions of HMGB1 and MMP-9 proteins in cholangiocarcinoma tissues were positively correlated(P<0.05).The positive expression rate of HMGB1 and MMP-9 protein in patients with portal vein infiltration,lymph node metastasis and poorly differentiated degree was higher than that in patients without portal vein infiltration,lymph node metastasis and moderately high degree of differentiation(P<0.05).Compared with patients with negative expression of HMGB1,the survival rate of patients with positive expression of HMGB1 was significantly lower(P<0.05).Compared with patients with negative expression of MMP-9,the survival rate of patients with positive expression of HMGB1 was significantly lower(P<0.05).Conclusion The abnormally high expression of HMGB1 and MMP-9 in primary cholangiocarcinoma is associated with poor prognosis of patients,and plays a synergistic role in the occurrence and progression of the disease.

关 键 词：胆管癌 高迁移率族蛋白1 金属基质蛋白酶-9 表达 病理 

分 类 号：R735.8[医药卫生—肿瘤]