Design and characterization of a bifunctional bybrid antibacterial peptide LLH for bactericidal/endotoxin neutralization effects  

作　　者：Rui-Qi Wang Chen-Peng Liu Hong-Ji Yao Jing-Yan Xiong Guo-Yu Li Yong Zhang 

机构地区：[1]Department of Department of Basic Medicine and Life Sciences,Hainan Medical University,Haikou 571199,China [2]Research Center of Pharmaceutical Engineering Technology,Harbin University of Commerce,Harbin 150076,China

出　　处：《Journal of Hainan Medical University》2022年第7期35-40,共6页海南医学院学报（英文版）

基　　金：Hainan University Scientific Research Funding Project(No.Hnky2019ZD-32)。

摘　　要：Objective:To design a bi-functional antimicrobial peptide with bactericidal and endotoxin neutralization activity,and explore its bactericidal properties.Methods:The LBP(86-99)peptides and HLF(1-11)peptides were connected by a GGGS flexible 4-peptide linker to obtain the bi-functional antimicrobial peptide,which was named LLH.The secondary structure characteristics of LLH were analyzed by Emboss software.The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of LLH against Escherichia coli ATCC25922 and DH5αwere determined by the microtiter broth dilution method.The bactericidal kinetics of LLH was characterized and its effect on endotoxin neutralization was determined.The hemolysis of LLH was evaluated.Results:LLH carried a positive charge of+9,exhibitedβ-folding andβ-corner structure,and had strong hydrophobicity.The MIC of LLH against Escherichia coli ATCC25922 and DH5αwas 4μM,and the MBC of LLH against Escherichia coli ATCC25922 and DH5αwas 8 and 4μM,respectively.LLH showed rapid bactericidal effects and significantly neutralized the endotoxin released in the sterilization process as well as reducing release of endotoxin.LLH showed no significant hemolysis at concentrations up to 400μg/mL.Conclusion:LLH produces dual effects of rapid sterilization and endotoxin neutralization,and does not induce significant hemolysis.

关 键 词：Hybrid antimicrobial peptides LINKER ENDOTOXIN BACTERICIDAL HEMOLYTIC 

分 类 号：R36[医药卫生—病理学]