龟龄集对AD小鼠皮层和海马Fas/FasL表达及神经元凋亡的影响  

Effect of Guilingji on the Expression of Fas/FasL and Neuronal Apoptosis in Cortex and Hippocampus of Alzheimer’s Disease Mice

作　　者：张怡[1,3] 权乾坤 李玺[1] 贾健[1] 雷亚玲 ZHANG Yi;QUAN Qiankun;LI Xi;JIA Jian;LEI Yaling(Department of Geriatrics,the Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an,710004,China;The Second Department of Encephalopathy,Shaanxi Traditional Chinese Medicine Hospital,Xi’an,712083,China;Department of Geriatrics,Xianyang Central Hospital,Xianyang,Shaanxi,712000,China)

机构地区：[1]西安交通大学第二附属医院老年病科,西安市710004 [2]陕西省中医医院脑病二科,西安市712083 [3]咸阳市中心医院老年病科,陕西省咸阳市712000

出　　处：《医学分子生物学杂志》2022年第3期213-218,共6页Journal of Medical Molecular Biology

基　　金：陕西省自然科学基础研究项目(No.2021JM-288);陕西省技术转让技术开发科研基金(No.SSX-GYY-20190605-161)。

摘　　要：目的探究龟龄集对阿尔茨海默病(Alzheimer’s disease,AD)小鼠皮层和海马Fas/FasL表达及神经元凋亡的影响。方法构建AD小鼠模型,小鼠随机分为对照组、模型组、多奈哌齐组及龟龄集低、中、高剂量组。Morris水迷宫检测干预前后小鼠的学习记忆能力;HE染色检测小鼠皮层、海马神经元病理学改变情况;TUNEL染色检测神经元凋亡情况;Western印迹及Real time PCR分别检测Fas、FasL的蛋白表达水平及mRNA表达水平。结果与模型组比较,龟龄集各组和多奈哌齐组小鼠学习记忆能力明显提高(P<0.05),皮层和海马神经元病理学损害及神经元凋亡改善(P<0.05),Fas、FasL蛋白和mRNA水平下降(P<0.05)。结论龟龄集可能通过抑制Fas/FasL表达抑制AD模型小鼠皮层、海马神经元凋亡,并改善其学习记忆能力。Objective To investigate the effects of Guilingji on the expression of Fas/FasL and neuronal apoptosis in cortex and hippocampus of mice with Alzheimer’s Disease(AD).Methods AD mouse model was constructed and randomly divided into the model group,donepezil group and low-,medium-and high-dose Guilingji groups.Morris water maze test was used to test the learning and memory ability of mice before and after intervention.HE staining was used to determine the pathological changes of cortical and hippocampal neurons.TUNEL staining was used to measure neuronal apoptosis.Western blotting and real-time PCR were used to detect protein and mRNA expression levels of Fas and FasL.Results The learning and memory ability of mice was significantly improved(P<0.05),the pathological damage and apoptosis of neurons in cortex and hippocampus greatly alleviated,and the protein and mRNA expression levels of Fas and FasL markedly decreased in Gulingji groups and donepezil group compared with model group(P<0.05 for all).Conclusion Gulingji may inhibit the apoptosis of cortical and hippocampal neurons and improve the learning and memory ability of AD model mice by inhibiting the expression of Fas/FasL.

关 键 词：龟龄集 阿尔茨海默病 凋亡 学习记忆能力 

分 类 号：R749.16[医药卫生—神经病学与精神病学]