着丝粒蛋白F表达对非小细胞肺癌生物学行为的影响和预后判断  

Effects of centromere protein F expression on biological behavior and prognosis of nonsmall cell lung cancer

作　　者：常彦祥[1] 金磊[1] 白雄雄 刘昊[1] 徐硕男 CHANG Yanxiang;JIN Lei;BAI Xiongxiong;LIU Hao;XU Shuonan(Department of Cardiothoracic Surgery,First Affiliated Hospital of Xi'an Medical University,Xi'an,710077,P.R.China)

机构地区：[1]西安医学院第一附属医院心胸外科,西安710077

出　　处：《中国胸心血管外科临床杂志》2022年第6期711-716,共6页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

基　　金：陕西省教育厅专项科研计划项目(18JK0674)。

摘　　要：目的研究非小细胞肺癌(non-small cell lung cancer,NSCLC)中着丝粒蛋白F(centromere protein F,CENPF)的表达和生物学功能,及其与预后的关系。方法通过生物信息学方法对Oncomine数据库、人类蛋白质图谱、Kaplan-Meier Plotter、STRING数据库、DAVID数据库进行数据检索和分析,明确CENPF在肺癌患者正常组织和癌组织中的表达情况,并对CENPF及其关联基因进行蛋白质互作网络分析、功能注释和通路分析。结果CENPF在肺腺癌组织中高表达,在正常组织中不表达。在NSCLC患者中CENPF低表达组的中位总生存期(overall survival,OS)显著长于高表达组。进一步进行亚组分析,腺癌患者低表达组中位无瘤进展生存期和OS均明显长于高表达组。CENPF及关联基因在生物过程中主要影响泛素连接酶K11修饰蛋白,在细胞成分中主要影响后期促进复合物(anaphase-promoting complex,APC)代谢,在分子功能中主要影响ATP结合,KEGG通路功能注释主要体现在对细胞周期的影响。结论CENPF参与了NSCLC的发生和发展过程,其在肺腺癌中的蛋白表达水平具有早期诊断和预后评估的价值。CENPF通过改变APC及泛素连接酶活性影响肺癌细胞增殖周期,提示CENPF基因可能成为NSCLC分子靶向治疗的潜在靶点。Objective To investigate the expression and biological function of centromere protein F(CENPF)in non-small cell lung cancer(NSCLC)and the association with prognosis.Methods Through retrieving and analyzing the bioinformatics data such as Oncomine database,Human Protein Atlas(HPA),Kaplan-Meier Plotter,STRING and DAVID database,the expression of CENPF in both normal tissues and cancer tissues of lung cancer patients was identified,and the protein interaction network analysis,functional annotation and pathway analysis of CENPF with its associated genes were carried out.Results CENPF was overexpressed in lung adenocarcinoma tissues,but not in normal tissues.The median overall survival(OS)of NSCLC patients with low expression of CENPF was significantly longer than that of patients with high expression of CENPF.Further sub-analysis showed that low expression group from lung adenocarcinoma patients had longer median disease-free survival and OS compared with high expression group patients.CENPF and its associated hub genes mainly affected the protein K11-linked ubiquitination in biological process,anaphase-promoting complex(APC)in cell composition,ATP binding in molecular function,and cell cycle in KEGG pathway.Conclusion CENPF is regulated in tumorigenesis and progression of NSCLC,and its protein expression level has the value of early diagnosis and prognosis evaluation in lung adenocarcinoma.It is suggested that CENPF gene can be a potential target for molecular targeted therapy of NSCLC.

关 键 词：非小细胞肺癌 着丝粒蛋白F 生物信息学 细胞周期 

分 类 号：R734.2[医药卫生—肿瘤]