大豆豆粕及赤小豆膳食补充对鼠伤寒沙门氏菌侵染小鼠肠道炎症的影响  

Effects of Dietary Supplementation of Soybean and Red Bean on Intestinal Inflammation in Mice Infected with Salmonella enterica serotype Typhimurium

作　　者：李萍[1] 苏佳丽 杜欣军[1] 王硕[2] LI Ping;SU Jiali;DU Xinjun;WANG Shuo(State Key Laboratory of Food Nutrition and Safety,College of Food Science and Engineering,Tianjin University of Science&Technology,Tianjin 300457,China;Tianjin Key Laboratory of Food Science and Health,School of Medicine,Nankai University,Tianjin 300071,China)

机构地区：[1]食品营养与安全国家重点实验室,天津科技大学食品科学与工程学院,天津300457 [2]天津市食品科学与健康重点实验室,南开大学医学院,天津300071

出　　处：《天津科技大学学报》2022年第3期12-20,36,共10页Journal of Tianjin University of Science & Technology

基　　金：国家重点研发计划项目(2018YFC1603800);河北省重点研发计划项目(20372801D)。

摘　　要：豆类作为人们日常生活中食用较多的一种作物,其在食源性致病菌侵染过程中的具体作用及机制并没有研究得很明确.通过建立鼠伤寒沙门氏菌(Salmonella enterica serotype Typhimurium)ATCC14028侵染小鼠引起肠道炎症的动物模型,设立淀粉正常组、淀粉侵染组、大豆豆粕侵染组、5%赤小豆侵染组和10%赤小豆侵染组.对4种饲料的膳食成分和功能成分及小鼠的生理生化指标如体质量、死亡率、结肠长度、粪便中的鼠伤寒沙门氏菌含量、血液炎症因子浓度、肠道切片进行研究,探究了大豆豆粕及赤小豆对肠道炎症的影响.研究发现,与喂食含有大豆豆粕的小鼠相比,食用不含有大豆豆粕饮食的小鼠更易受到致病菌的侵染,引发更严重的肠道炎症.用赤小豆进行膳食补充则可以降低粪便中鼠伤寒沙门氏菌的含量,下调血液中促炎因子的水平,上调抗炎因子的水平,保持肠道形态结构完整,使其生理生化指标接近未受侵染的小鼠.因此,在日常饮食中,长期食用适量的豆类可以使人体在致病菌侵染时得到一定的保护.As a daily food,the specific role and mechanism of legumes in the infection process of foodborne pathogens has not been clearly studied.In this article,an animal model of intestinal inflammation induced by the infection of Salmonella enterica serotype Typhimurium ATCC14028(S.Typhimurium)in mice was constructed.And four groups including starch control group,starch infection group,soybean meal infection group,5%red bean infection group and 10%red bean infection group were set to investigate the role of soybean and red bean components in intestinal inflammatory regulation.The dietary and functional composition contents and physiological and biochemical indexes of mice,such as body weight,mortality,length of the colon,the content of S.Typhimurium in feces,the concentration of inflammatory factors in blood,and histopathological observation of the intestine,were determined to explore the effects of soybean and red bean on intestinal inflammation.Results indicated that,compared with the mice group fed with soybean meal,the mice group fed with the diet that did not contain soybean meal were more susceptible to infection by pathogenic bacteria,thus causing more severe inflammation of the gut.Red bean in dietary supplement could alleviate intestinal inflammation,reduced the content of S.Typhimurium in feces,decreased levels of pro-inflammatory factors in the blood,increased levels of anti-inflammatory factors,maintained the integrity of the gut morphology,and made the infected mice have the similar physiological and biochemical indicators as uninfected mice.Therefore,in the daily diet,long-term consumption of a moderate amount of beans can protect the human body to a certain extent,when it is infected by pathogenic bacteria.

关 键 词：大豆豆粕 赤小豆 鼠伤寒沙门氏菌 肠道炎症 炎症因子 

分 类 号：TS214[轻工技术与工程—粮食、油脂及植物蛋白工程]