深黄伞形霉与黑曲霉混合固态发酵麸皮积累油脂  

Lipid accumulation by mixed solid state fermentation of Umbelopsis isabellina and Aspergillus niger with wheat bran as substrate

作　　者：郑潇 冯豪[1] 于洁 王子硕 周化岚[1] Sarman Oktovianus Gultom 张建国[1] ZHENG Xiao;FENG Hao;YU Jie;WANG Zishuo;ZHOU Hualan;Sarman Oktovianus Gultom;ZHANG Jianguo(Institute of Food Science and Engineering,School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;Department of Agricultural and Biosystems Engineering,Papua University Manokwari,Papua Barat,Indonesia)

机构地区：[1]上海理工大学健康科学与工程学院,食品科学与工程研究所,上海200093 [2]Department of Agricultural and Biosystems Engineering, Papua University Manokwari, Papua Barat, Indonesia

出　　处：《工业微生物》2022年第2期17-24,共8页Industrial Microbiology

基　　金：上海市国际科技合作基金项目(19230742900)。

摘　　要：深黄伞形霉是一种富油丝状真菌,油脂含量可达86%。并且深黄伞形霉油脂中富含油酸,亚油酸和γ-亚麻油酸等不饱和脂肪酸。黑曲霉是食品安全性好的工业微生物菌株,可高效产出纤维素酶、纤维二糖酶、木聚糖酶、淀粉酶、糖化酶、果胶酶等多种酶,用来水解多种食品废弃物。因此将深黄伞形霉和黑曲霉混合以麸皮为底物进行固态发酵,促进麸皮中糖的释放以及油脂产量的提高,并且优化了固态发酵的条件,结果表明固态发酵的最适初始水分含量为75%、最适接菌量为107 CFU/g麸皮、最适孢龄为7 d、最适菌种比例为黑曲霉∶深黄伞形霉=1∶1,在此条件下可获得59.04 mg/g麸皮的油脂产量。本研究为利用生物质积累油脂提供了思路。Umbelopsis isabellinais is an oil-rich oleaginous filamentous fungus with its oil content up to 86%in vivo,which is rich in oleic acid,linoleic acid,γ-linoleic acid and other unsaturated fatty acids.And,Aspergillus niger is an industrial microbial strain with good food safety,which can efficiently produce many kinds of enzymes such as cellulase,cellobiase,xylanase,amylase,glycosylase,pectinase and so on for the hydrolysis of a variety of food wastes.Therefore,the mixed culture of Umbelopsis isabellina and Aspergillus niger was used for solid state fermentation with wheat bran as substrate to promote the release of sugar in wheat bran and the increase of lipid yield in this paper,and several parameters of mixed solid state fermentation were optimized as follows:the optimum initial water content was 75%,the optimum inoculation amount was 10 CFU/g wheat bran,the optimum spore age was 7 d,and the optimum strain ratio of A.niger to U.isabellina was 1∶1.Under the optimized conditions,the highest lipid yield of 59.04 mg/g bran was obtained.This study provided an idea for the use of biomass to accumulate lipid.

关 键 词：深黄伞形霉 黑曲霉 固态发酵 油脂产量 脂肪酸 

分 类 号：TS2[轻工技术与工程—食品科学与工程]