环状RNA肌球蛋白轻链激酶靶向微小RNA-497-5p调控结肠癌细胞的增殖、迁移和侵袭  

CircMYLK regulates the proliferation,migration and invasion of colon cancer cells by targeting miR-497-5p

作　　者：宋德顺[1] 程华[1] SONG Deshun;CHENG Hua(Department of Endoscopy,Qingdao Eighth People’s Hospital,Qingdao,Shandong 266100,China)

机构地区：[1]青岛市第八人民医院内镜诊疗科,山东青岛266100

出　　处：《安徽医药》2022年第7期1355-1360,共6页Anhui Medical and Pharmaceutical Journal

摘　　要：目的 探讨环状RNA肌球蛋白轻链激酶(circMYLK)对结肠癌细胞增殖、迁移和侵袭的影响和可能机制。方法 本研究2019年3月至2020年1月进行。实时荧光定量PCR(RT-qPCR)检测检测正常结肠上皮细胞(NCM460)和结肠癌细胞(SW480、SW620、Caco-2)中circMYLK和微小RNA-497-5p(miR-497-5p)表达水平。将circMYLK小干扰RNA(si-circMYLK)、miR-497-5p模拟物分别转染SW480细胞,采用细胞计数试剂盒(CCK-8)、Transwell实验分别检测干扰circMYLK或过表达miR-497-5p对SW480细胞增殖、迁移和侵袭的影响。双荧光素酶报告基因实验和RT-qPCR确定circMYLK对miR-497-5p的调控作用。结果 与NCM460细胞比较,结肠癌细胞中circMYLK表达(1.00±0.06比3.39±0.23、2.31±0.24、2.98±0.18)显著升高,miR-497-5p表达(1.00±0.08比0.36±0.04、0.63±0.05、0.54±0.05)显著降低(P<0.05)。干扰circMYLK表达后SW480、细胞活力、迁移和侵袭细胞数显著降低(P<0.05)。过表达miR-497-5p后SW480细胞活力、迁移和侵袭细胞数显著降低(P<0.05)。circMYLK靶向负性调控miR-497-5p表达。抑制miR-497-5p表达能够逆转干扰circMYLK对SW480细胞增殖、迁移和侵袭的影响,恢复细胞活力、迁移和侵袭能力(P<0.05)。结论 结肠癌细胞中circMYLK呈高表达,干扰circMYLK通过靶向miR-497-5p能够抑制结肠癌细胞增殖、迁移和侵袭。Objective and invasion of colon cancer cells and its possible mechanism.Methods This study was conducted from March 2019 to January 2020.Real-time fluorescence quantitative PCR(RT-qPCR) was used to detect the expression levels of circMYLK and miR-497-5p in normal colon epithelial cells(NCM460) and colon cancer cells(SW480,SW620,Caco-2).The circMYLK small interfering RNA(si-circMYLK)and miR-497-5p mimics were transfected into SW480 cells,respectively.The cell counting kit(CCK-8) and Transwell experiments were used to detect the effects of interfering with circMYLK or overexpressing miR-497-5p on proliferation,migration and invasion of SW480 cells.The dual luciferase reporter gene experiment and RT-qPCR were used to determine the regulatory effect of circMYLK on miR-497-5p.Results 0.18) in colon cancer cells was significantly increased,while the expression of miR-497-5p(1.00 ± 0.08 vs.0.36 ± 0.04,0.63 ± 0.05,0.54 ± 0.05) was significantly reduced(P<0.05).After interfering with circMYLK expression,the cell viability,migration and invasion cell numbers of SW480 were significantly reduced(P<0.05).After overexpression of miR-497-5p,the cell viability,migration and invasion cell numbers of SW480 were significantly reduced(P<0.05).circMYLK could target and negatively regulate miR-497-5p expression.Inhibiting the expression of miR-497-5p could reverse the effect of interfering with circMYLK on the proliferation,migration and invasion of SW480 cells,and restore cell viability,migration and invasion ability(P<0.05).Conclusions pressed in colon cancer cells.Interfering with circMYLK can inhibit the proliferation,migration and invasion of colon cancer cells by targeting miR-497-5p.

关 键 词：肌球蛋白轻链激酶 结肠肿瘤 微小RNA-497-5p 增殖 迁移 侵袭 

分 类 号：R735.35[医药卫生—肿瘤]