血管生成拟态在翼状胬肉进展中的作用及可能机制  

Role of vasculogenic mimicry in the development of pterygium and its possible mechanism

作　　者：贺梦璇 唐秋阳 张俊芳[1] 杨铃[1] 秦柏[1] 顾宏卫[1] 管怀进[1] 石海红[1] HE Mengxuan;TANG Qiuyang;ZHANG Junfang;YANG Ling;QIN Bai;GU Hongwei;GUAN Huaijin;SHI Haihong(Department of Ophthalmology,Affiliated Hospital of Nantong University,Nantong 226001,Jiangsu Province,China)

机构地区：[1]南通大学附属医院眼科,江苏省南通市226001

出　　处：《眼科新进展》2022年第6期474-479,共6页Recent Advances in Ophthalmology

摘　　要：目的研究血管生成拟态(VM)在翼状胬肉进展中的作用及其与基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)和血管内皮生长因子(VEGF)表达的关系,探讨VM与翼状胬肉进展的相关性及可能机制。方法筛选符合条件的翼状胬肉标本,进行胬肉分期(静止期/进展期)、分型(初发型/复发型)及临床特征指标采集。采用CD31/PAS双重染色检测不同类型翼状胬肉中VM的形成差异。以初发型进展期翼状胬肉为研究对象,HE染色观察病理特征,免疫组织化学染色及Western blot检测MMP-2、MMP-9及VEGF的表达,分析VM与初发型进展期翼状胬肉临床病理特征及MMP-2、MMP-9、VEGF的相关性。结果静止期翼状胬肉VM阳性率为15.38%,进展期翼状胬肉VM阳性率为68.50%,组间差异具有统计学意义(P=0.000);初发型翼状胬肉VM阳性率为40.80%,复发型翼状胬肉VM阳性率为81.48%,组间差异亦具有统计学意义(P=0.000)。VM与进展期及复发型翼状胬肉均呈正相关(均为P<0.05)。VM阳性的初发型进展期翼状胬肉组织,其血管分级、上皮细胞层数、杯状细胞数、成纤维细胞数、血管数、炎症细胞数、纤维化程度、MMP-2、MMP-9及VEGF的表达均明显高于VM阴性者,而变性程度低于VM阴性者,差异均有统计学意义(均为P<0.05)。初发型进展期翼状胬肉VM与翼状胬肉血管分级、上皮细胞层数、杯状细胞数、成纤维细胞数、血管数、炎症细胞数、纤维化程度、MMP-2、MMP-9及VEGF的表达均呈正相关,与胬肉变性程度呈负相关(均为P<0.05)。结论翼状胬肉组织中存在VM结构,可作为其血供途径之一,VM可促进翼状胬肉的进展,MMP-2、MMP-9及VEGF可能是翼状胬肉VM形成的分子机制。Objective To investigate the role of vasculogenic mimicry(VM)in the development of pterygium and its correlation with the expression of matrix metalloproteinase-2(MMP-2),matrix metalloproteinase-9(MMP-9)and vascular endothelial growth factor(VEGF),and explore the correlation between VM and pterygium progression and its possible mechanism.Methods Qualified pterygium specimens were screened,and their staging(quiescent/progressive stage),typing(initial onset/relapsing type)and clinical characteristics were collected.CD31/PAS double staining was performed to detect the difference in VM formation in different types of pterygiums.Initial-onset progressive pterygiums were selected for further research.Their clinicopathological features were observed by Hematoxylin and Eosin staining,and the expression levels of MMP-2,MMP-9 and VEGF were measured by immunohistochemical staining and Western blot.The relevance of VM to the clinicopathological features and the expression levels of MMP-2,MMP-9 and VEGF was analyzed.Results The positive rate of VM was 15.38%in quiescent pterygiums and 68.50%in progressive pterygiums(P=0.000),40.80%in initial-onset pterygiums and 81.48%in relapsing pterygiums(P=0.000).VM was positively correlated with progressive and relapsing pterygiums(both P<0.05).The vascular grading,the numbers of epithelial cell layers,goblet cells,fibroblasts,blood vessels and inflammatory cells,the degree of fibrosis,and the expression of MMP-2,MMP-9 and VEGF in initial-onset progressive pterygiums with VM positive were significantly higher than those with VM negative,while the degree of degeneration was observably lower(all P<0.05).VM in the initial-onset progressive pterygiums was positively correlated with the vascular grading,the numbers of epithelial cell layers,goblet cells,fibroblasts,blood vessels and inflammatory cells,the degree of fibrosis,and the expression of MMP-2,MMP-9 and VEGF,and negatively correlated with the degree of degeneration(all P<0.05).Conclusion VM exists in pterygiums and can be used as one

关 键 词：翼状胬肉 血管生成拟态 基质金属蛋白酶-2 基质金属蛋白酶-9 血管内皮生长因子 

分 类 号：R777.33[医药卫生—眼科]