Valerian (Valeriana officinalis) extract inhibits TNF-α and iNOS gene expression in mouse LPS-activated microglial cells  

作　　者：Hashem Marawne Reza Mohammadhassan Zahra Mohammadalipour Sedigheh Ahmadpour 

机构地区：[1]Amino Techno Gene Virtual Private Institute(NGO),Tehran 1495845882,Iran

出　　处：《Traditional Medicine Research》2022年第5期65-70,共6页TMR传统医学研究

摘　　要：Background:Inflammation and damage to neurons and other cells in the nervous system can cause disorders of the central nervous system.Microglial cells are activated by pathogen infection and injury to release nitric oxide.Valerian(Valeriana officinalis)has been used as a sedative for the treatment of neurological diseases.This study evaluated inflammation of microglial cells and tumor necrosis factorαand induced nitric oxide synthetase gene expression influenced by valerian extract.Methods:Microglial cells were isolated from mice.Lipopolysaccharide(1 ng/mL)was used to induce inflammation and nitric oxide production in cells for an hour.The inflamed cells were then treated with different concentrations(0.1,0.5,2.5,20,and 50μL/mL)of valerian alcoholic extract for 1 and 24 h.nitric oxide production and tumor necrosis factorαand induced nitric oxide synthetase gene expression were determine by Griess assay and real-time polymerase chain reaction,respectively.Results:The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed no toxicity in several concentrations of the valerian extract.In addition,concentrations from 0.1 to 2.5μL/mL significantly reduced inflammation and nitric oxide production in mouse microglial cells to levels observed in control samples.Furthermore,tumor necrosis factorαand induced nitric oxide synthetase gene expression decreased when 2.5μL/mL extract was used.Conclusion:Based on these results,it can be concluded that 2.5μL/mL valerian alcoholic extract is effective as a candidate alternative medicine for reducing inflammation and nitric oxide production and consequently,the inflammatory symptoms of neurodegeneration.

关 键 词：microglial cells nitric oxide Valeriana officinalis TNF-Α INOS 

分 类 号：R73[医药卫生—肿瘤]